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77-33 49,6 50 50 Bacillus sp. 77-1 49 49,5 51 77-47 50 50 51,3 59-1 50 49,7 50 50,59-2 48,2 49 49 59-3 51 50,5 50 49,59-8 49 50 50 Bpaae poopao ocyec a epao oe c aeao eee 3-x cyo, a a Bacillus sp. 77-1 eee 1 cyo (ooao A.A., eyxoa H.., op B.B. // X. 2000).

2. cceoae cocaa cpe a apyy aoc epcex poopao o poeeo cceoae cocaa pax aoee ocyx cpe a apyy aoc poc poopao (a. 3).

Taa Be cocaa cpe a poc aoc poopao Teepaypa ypoa 300C, eepaypa pea 30 C, pe pea 24 aca, oepa cycpaa 10 / a Poc* Apya aoc, / CP-1 CP-2 CP-3 CP-1 CP-2 CP-77-33 1 2 3 0,77 1,4 1,77-47 1 2 3 0,82 1,61 1,59-1 1 2 3 0,41 0,87 0,59-2 1 2 3 0,28 0,75 0,59-3 1 2 3 0,79 1,7 1,59-8 1 2 3 0,17 0,58 0,57-11 1 2 3 0,88 1,8 1,peae: CP-1 epaa cpea c aeao, CP-2 epaa cpea c aeao, ooaea poe aoao (200 /) eoo (200 /), CP3 cyco; * - poc poopao oea aax (0 ocyce poca, 1 ca poc, 2 xopo poc, 3 o poc) oyee peya (c. a. 3) oaa, o a cpeax CP(aapoaoe cyco) CP2 (epa o c aeao, ooae poe aoao eoo) aac ao poc oacc, co oepe, aoc epeo y cex poopao, paex a (CP-2) aapoao cyce (CP-3), aapyeo pea paec e eec, a p paa a cpee CP(epa o c aeao) o oeeo cyeceoe cee apye aoc. o peoaae oooc cooa aece pocooo cycpaa pooo pya ocyx eec, oeceax coy copoc poca poopao.

3. Papaoa oaaaopo apoa a ocoe eo poopao B peyae cceoa e apoa eaoa-2 o ycaoeo, o ce pa ycox cpecc-eoa pocxo eeo, o opaae eo cooae oye eaoa-2 peapax ex. Oco opaee copoc cea ec eooe oeco aaaopa, ocoo peaoy cec cocae aapooo oa, c c eca poecca eoxoo o papaoa coco ece opao ox oec oaaaopa.

Oao oe cyecoa pya pa ocyc aoc cpo oacc o aa oepxoc oaa eo c opaec pacopee, ocoy p epeea oacca caaec pye pay, o apye accooee poecc. poe oo, ae oo co opy e ae oe pecoa pooe cycpaa ey aoec c ypeox epeo.

3.1. cceoae oeoa oacc aeoo a ee poay apyy aoc o poeeo cpaee aoc oacc, peapeo oeoeo opaoo aeoo, eopaoao oacc p poe cox po ycyco co apoa eaoa-2 aeao (a. 4).

Taa Aoc aeoox opoo p poe aeaa, yaeaa aeaa apoa eaoa-Teepaypa 30 0C, oepa cycpaa 10 /, pe pea 24 aca poaa aoc, Apya aoc, / / a Heopaoaa Opaoaa Opaoaa oacca oacca oacca 77-1 1,8 1,9 2,77-33 1,5 1,65 2,77-47 1,5 1,5 1,59-1 1,1 1,23 1,59-2 1,3 1,35 1,59-3 1,1 1,27 2,59-8 0,3 0,45 0,B peyae oo coc, o oeoae eo poopao aeoo e oo yeae copoc poa yaax po, o ae ooe eo poo apoae eaoa-(c. a. 4), o oe cooao aaa pea apaoo apoa paeecx cpo opaecx cpeax.

3.2. paa cxea oye oaaaopo apaoo apoa cpo a peoea paa cxea pooca oaaaopo apoa cpo a ocoe eex ao (pc. 2), oopa ae ce paae yyp poopao y cocoo epeepe 1 a cyce eee 3 e (pHa.=7,0, t=30 0C). aee paea oacca oeec o yypao oc epypoae, oaec oca 0,05 M yepo pH 7,0 o ocao cpe (eoc 3), oyoaec a epye 4 opaaaec aeoo (aeo : oacca 3:1) eoc 5. Opaoaa oacca ocooaec o aeoa a epye 6 cyc p oao eepaype.

opaoa opaoa yep aeo c oyx yep oacca 2 aeo a oa peeepa yypaa oacca oc oaaaop oeoea oacca a xpaee Pc. 2. paa cxea oye oaaaopo apaoo apoa cpo:

1 epeep yoo ypoa; 3,5 eoc, 2,4,6 epy; 7 ayyo-cy a; CB cep oyx; TH - eooce cooae peoeo cxe ooe oya 5 7 oaaaopa e oeoeo oacc c 1 pa o cpe (cyca).

4. cceoae caoc oaaaopa p eo xpae Coxpaee aoc p eo xpae ec o ocox peoa, peex oaaaopa p x cooa poex acaax. B c c o poeeo cceoae caoc oaaaopa p xpae a e eopaoao oyoao oacc, a e opaoao aeoo oacc eee 9 ece p eepaype 15 0C.

Taa acoc apye aoc aopoeo cpo oacc oeoex eo o eoc xpae Teepaypa pea 30 0C, pe pea 24 aca, oepa cycpaa 10 / Apya aoc eo, *% Xpaee e Xpaee e aeoooo a aopoeo oacc opoa 1 3 6 9 1 3 6 ec eca ece ece ec eca ece ece 77-47 100 95 60 25 100 110 100 77-33 100 102 75 30 100 101 98 77-1 100 - 35 100 100 99 59-1 100 87 70 20 100 101 102 59-2 100 90 50 10 100 99 100 59-3 100 92 65 40 100 100 99 59-8 100 - - 30 100 104 101 peae: * a 100 % pa aoc cee oacc aeoooo opoa B peyae oo o ycaoeo, o opaoaa aeoo oacca ec oee cao o cpae c eopaoao oe coxpae co aoc (a. 5).

Ta opao, opaoa oacc aeoo oae caoc oaaaopa, e ca p o e aoc. poe oo, ooa opaoa ooe ye copoc pea, a a peyae opao eo aeoo oaec poaeoc x eoo epa, cece eo cycpa caoc oee ocy ypeo epea. Bce o ooe oee eo cooa eee poopa aece oaaaopo poeccax apoa opx cpo opaeco pacopee.

5. cceoae yco apoa eaoa-2 oeoe ea Ha ceeoc poecca xo poya cyeceoe e oe oaa a opaecoo pacope, a ppoa apyeo aea. B co oepe, eepaypa pea ae oaae aeoe e a aoc oaaaopo. B c c o poeeo cceoae x aopo a apoae eaoa2 aeao pcyc eo poopao, poex peapey opaoy aeoo c e oe eoc pacopa eaoa-2.

5.1. cceoae eepayp a apoae eaoa-eee apye aoc oaaaopo p pax eepaypax poecce apoa eaoa-2 aeao ecae pecaeo a pc. 3.

2,77-77-77-1,59-59-59-59-0,15 20 25 30 35 40 45 Teepaypa, C Pc. 3. Be eepayp a aoc oaaaopo apaoo apoa cpo oepa cycpao 10 /, pe pea 24 .

Macaa aoc y oca papaoax oaaaopo c 50% oepce cycpaa ocaec p 35 37 0C, a a 7747 59-1 po acay aoc p 32 40 0C cooeceo.

5.2. Be ppo opaecoo pacope a apoae eaoa-Hecop a o, o oco epeo xopoo paoa pax opaecx pacopex (ao, 1986; Gray, 1990), pacope oe oaa a ooeoe, a opaeoe oece a aoc caoc papaaaex oaaaopo.

Oxapaepoa opoc pacope oo p oo ae ooceoo oea Log P (ao, 1989; Laane, 1987). B oe cyae pacopex c eo Log P < 2.0 poec caa oaaeca aoc, a pacopex c eo Log P > 4 coa aoc.

B a. 6 oaao e pax opaecx pacopee a poecc apoa eaoa-2.

Aoc, / Taa Be ppo opaecoo pacope a apoae eaoa-Teepaypa 30 C, pe pea 24 , oepa cycpao 10/, oepa oeoeo oacc 30 / Pacope Log oepc eaoa-2, % P 77-33 77-47 77-1 59-1 59-2 59-3 59-ooa 4,5 50 51 50 50 49 50 ea 4,0 50 50 50 49 50 51 eca 3,5 50 50 50 48,9 49,7 50 ea 3,0 49 50 51 50 50 50 Toyo 2,5 32 40 23 30 18 12 eo 2,0 37 33 12 28 15 10 Xopoop 2,0 21 10 12 25 5 13 Tepapoypa 0,49 2,5 4 1,7 0 0 3 Aeo -0,23 0 0 0 0 0 0 Aeop -0,33 0 0 0 0 0 0 o p 0 0 0 0 0 0 B ax pacopex, a ooa, eca, ea, ea c eo Log P > 3.0, aaac coa oepc cycpaa, o pe a xopoope, eoe oyoe oepc eaoa-2 caac, a pacopex c eo Log P < 2.0 aoc oaaaopo paec ocycoaa.

5.3. Be cpoe apyeo aea a pacopa eaoa-cceoae ppo oopa ao py a pacopa eaoa-2 ecae ea, opaoa aeoo, oaao, o aoee oxo oopo ec aea, c oop ce a oeppoa cp a 49- 50 %.

Taa Be ppo apyeo aea a pacopa eaoa-Teepaypa 30 C, pe pea 24 , oepa cycpao 10 /, oepa oeoeo oacc 30 / oop oepc, % ao py 77-1 77-33 77-47 59-1 59-2 59-3 59-Baea 49 49,6 50 50 48,2 51 yaea 10,5 1,4 4,7 6,7 7,8 5 aea 9 2,1 1,5 4 6 3,4 4,cyca coa 3 0 4 7 5,5 3 oaca -a 11,5 19 13 18 10 8 Baepaoa -a 7,2 15 14 4 13 11 Oeoa -a 10 0 5 19,5 15 11,5 25,cceoae epa eaoa-2 a opaec coa, a oeoa, aepaoa, oaca ycyca, oaao, o paec ce cceyee a coco aapoa epa eaoa-2 yaa opaec coa.

OCOR OH H2O RCOOH e R: CH3, C4H9, C5H11, C9H19, C8H17CH=CH(CH2)Oao ce a oaa ooo y cee pepae eaoa-2 (Ta. 7). cooae ooo acopea eoa 4 A c e yae opayec peyae pea o e peo cyeceoy yee oepc cpa.

6. cceoae caoc oaaaopo poecce apoa C e oe caoc papaaaeoo oaaaopa a cceoaa cocooc oacc, opaoae o apoa, ocyec oope pacopa cycpaa, ocoo a pa epoaao oepa.

2,59-59-59-59-1,77-77-77-0,1 2 3 4 5 Pc. 4. Copoc apoa eaoa-2 aeoo opoa eee 6 o Teepaypa 30 0C, pe pea 24 aca, oepa cycpao 10/, oepa oeoeo oacc 30 / caoeo, o ce oaaaop po cocooc aapoa apoae eaoa-2 eee 6 o c ocee 50 % oepc cpa (pc. 4). Oao copoc apoa eaoa-2 6 y aae cac y cex poopao, ocoeo y ao 59-1, 59-2 59-8. Booo, o pocxo -a oceeo aa Aoc, / 1, 59-2 59-8. Booo, o pocxo -a oceeo aa apyx epeo o ece a opaecoo pacope (ecaa), a opayxc poyo pea.

Ta opao, aoee epce aa, aapy apaoe apoae cpo, c a 77-33, 77-47, 59-3 Bacillus sp. 77-1, oaae oe aoc caoc p oopao cooa, o cpae co aa 59-1, 59-2 59-8.

7. ea poopao cceoae aoex coc C e ea oyex peyae cpa aoee ax ao ye x ocoe opooece ooo-oxece coca. ae cceoa oaa, o oopae a 77-33, 77-47 59-3 aoee o oca aep poa Psudomonas sp.

ca ao a aoeoc, oee copya cya oo pya e eoea, oaa, o o e oaa aoe eoea ox coca.

8. Papaoa eoa eecoo paee eaoa-p papaoe eoa eecoo paee eaoa-2 a cooaa paa cxea, pecaea a pc. 5, oopa ae ce: pacopa cycpaa peaope 1, ya oeaec pacope eca, oopo pacopec cycpa aea, oce epec c o 10-15 %. oaaaop oaec pacea 30 /. Pea pooc eee 8 aco p 32-37 0C. oce aepe pea oaaaop oeec o peaoo cpe epypoapacope cycpa e oaaaop pacope oaaaop e e cp p Pc. 5. paa cxea oye (S)-eaoa-coe ooae: 1 peaop, 2 epya, 3,5,6 ayy cape, 4 ooa BX e 2. Peaoa cec yapaec a ayyo-poopo capee 3.

Paeee cpoo po pa ocyecec eoo BX ( eca:aea 1:1) 4. pa, coepae cp p, yapac o ayyo 5 6.

o peeo cxee papaoa eo oye oec ax (S)-(+)-eaoa-2 (R)-(-)-eaoa-2, oope c ae oec a coa.

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