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a 6 o, o ae oaaaop oy cooac oee 6 o pacopa. Aoc oaaaopa a ocoe aa Bacillus sp. 77-1 oce 6 o e eec, a cyae oaaaopa a ocoe aa Rhodococcus sp.78-5 aae eaeo.

peapa ce ( (S)-op-yaoa 3.4.3. R)-op-yaeaa oyee peapax oec op-yaeaa opyaoa poo o pao cxee pecaeo a pcye 5.

Taa Bxo oeca coa poyo poa op-yaeaa aa Bacillus sp. 77-1 Bacillus sp. 79-54 oax ycox oaaaop (R,S)- oe- Ocaoa (S)-(-)-op- (R)-(+)- opy opy- co aoc, yao aea a acca, aea, o % xo, ee,% xo, ee,% Bacillus sp. 30 42 6 90 17,8 98 17 77-Bacillus sp. 30 42 6 95 18,2 96 18,5 79-p eeco paee 42 paeecoo op-yaeaa eee 6 o aa Bacillus sp. 77-1 Bacillus sp. 79-54 oye (S)-(+)-op-yao (R)-(-)- op-yaea c xoo 80-88% (o eopeec oooo p ao oepc pa) oeco coo 96-99% ee (a. 7). Ocaoa aoc oaaaopo oce 6 o pacopa coxpaec a ypoe 90-95%.

3.5. Papaoa peapax eoo eecoo paee paeecx -3-ocypaa op-yaeaa opaeco cpee eco, o a poopao oy aapoa e oo po po, o pea x pacepa ( o ce pea aooa aoa po), poeax opaeco ae. p o pe cyae pea epa oy oaac oee e oye oec ax coee. poe oo, poeee pea opaeco ae oe oe aay ee eex oec ax coee, opayxc p pacopa. B c c o eecoopao cceoa oooc pee papaoax oaaaopo peax aooa aoa paeecx cece cceyex po. B acoe paoe ye pea aoceeoo aooa -3-ocypaa aoa opyaoa ecae.

3.5.1. Aoo -3-ocypaa -yaoo yee aooa -3-ocypaa -yaoo peopaoa aeoo ea ao Bacillus sp. 77-1 Rhodococcus sp. 785 oaao, o cee eo pepae (~50%) xo poya (100% o eopeec oooo p ao oepc pa) e ac o oepa cycpaa. o yaae a o, o opaec pacope poy pea e oaa pyeo ec a epey ccey ao.

a H HO H OH OH (R)-a COOBu + COOEt COOEt (R)-(-)-y-3-ocypa (S)-(+)--3-ocypa (R,S)--3-oc EtOH ypa H H OH HO + COOEt + COOBu BuOH a (R)-(-)--3-ocypa (S)-(+)-y-3-ocypa yao-(S)-a poeee pacopa eee 6 o e o aeoo ce aoc oaaaopo, o oop o x caoc opaeco pacopee.

Taa Bxo oeca coa -3-ocypaa, oyeoo aooo yaoo-1 aa Bacillus sp. 77-1 Rhodococcus sp.78-5 ecae pe 5 .; aaa oepa cycpaa 6,6 / (cycpa : peae, 1:1,1).

oaaaop (R,S)-- oe- Ocaoa (R)-(-)--3- (S)-(+)- -3 3-ypa, co aoc, ocypa ocypa o % a acca, xo, ee, % xo, ee, % Bacillus 30 39,6 6 84 16,63 97 - sp. 77-Rhodococ- 30 39,6 6 90 - - 16,8 cus sp.78-aapye aa Bacillus sp. 77-1 Rhodococcus sp.78-5 aoo 39,6 paeecoo -3-ocypaa yaoo-1 ecae eee 6 o (6,6 pa a 1 ) c ocey paeee poyo eoo oco xpoaopa oo oy poy c xoo 80-90% (o eopeec oooo p ao oepc pa) oeco coo 97-100% ee (a. 8).

3.5.1. paa cxea eecoo paee paeecx cece -3-ocypaa op-yaeaa oyee peya oo papaoa ayo oocoay pay cxey eecoo paee paeecx po opaeco ae, oopa ae ce 3 ca: oyee oaaaopo, eecoe paeee paeao ecae, eee oyex oepo peaoo cec (pc. 6).

eca aeo oacca oaaaop I. op-y aea c 2 aepaoa coa ypa II. -3-oc yao yypaa aeo oc oaaaop eca:aea 3:8 eca pa pa 10 12 (R)-opI.

(S)-opII. (S)--3-ocypa I.

II. (R)-y-3-ocypa yaea yaepa (R)--3-ocypa (S)-y-3-ocypa 1-epeep; 2,4,6-epy; 3-oc c eao; 5-peaop; 7,12,13- ayyopoope cape; 8,10,11,14,15 oc; 9- ooa X, c cep oyx, eooce.

Pc. 6. paa cxea eecoo paee opyaeaa -3-ocypaa opaeco ae.

Ca oye oaaaopo aaoa ca oye oaaaopo cxee pecaeo a pcye 5. Ha 2 ca pooc eecoe paeee paeecx cece op-yaeaa aoo aepaoo coo (1:1,1) -3-ocypaa aooo yaoo-1 (1:1,1) ecae (aaa oepa cycpaa 50 M/; t= 30-350C, acoc o oaaaopa oepa oopoo 30 /) P eee 5 aco 5 c ocey oeee peaoo cec o o aaaopa . oaaaop oe cooac oopo e eee 6 o pacopa e ep aoc.

BP Ha 3 ca eca ooec a 7. oyea cec oepo paeec eoo X a aoeo caee (e eca:

E aea, 3:1). pa coepae oece oep copac oyx opaoa E BP 10 11 aee e ooec a 12 13, a poy ocya E E 14 15.

3.5.3. Ao op-yaeaa aepaoo coo yee aoa op-yaeaa (aaa oepa 2,5 5 /) aepaoo coo eee 6 o oaao, o aoc aa Bacillus sp. 79-54 oepc pa, paec e eec, oa a y aa Bacillus sp. 77-1, aoc aeo yeaec c a o, o oe oycoeo aae epea cece oec aepaoo co.

Et Me Me OCOBu + C C + BuCOOH OAc H Et H OAc (R,S)- (R)-(-)- (S)-(+)aepaoa op-yaea op-yaea coa op-yaepa.

.

peee papaoao pao cxe (pc. 6) paee paeecx po opaeco ae ooo oy (R)-(+)-opyaea c xoo 87% (o eopeec oooo p ao oepc pa) oeco coo 99% ee.

Taa Bxo oeca coa (R)-(+)-op-yaeaa, oyeoo apa aoo paeecoo op-yaeaa aepaoo coo, aapyeoo opaoa aeoo ea aa Bacillus sp. 79-54 ecae pe 5 .; aaa oepa cycpaa 5,8 / (cycpa : peae, 1:1,1).

oaaaop (R,S)- o- Ocaoa (R)-(+)- opyaea opy- eco aoc, a acca, aea, o % xo, ee,% Bacillus 30 34,8 6 88 15,1 sp. 79-B peyae cpaeoo aaa pax cxe paee oo (pc. 5) opaeco (pc. 6) cpeax ycaoeo, o oyee oec ax ocaox cycpao op-yaeaa 3-ocypaa peoeee poo ecae cece ee pyooc oe eoc paee.

3.6. cceoae ceecoo oeaa oaaaopo eoopx oex peax opaeco pacopee Moe a, coaco epayp a, oaa poo cycpao ceoc, oy aapoa apy c poo, pye pea. B c c a cceoaa oooc aoceeoo aaa oex peax c py cycpaa.

ye pea aooa aooa ooo pa eapoo co aooa N-3E-ee-2--opo oyaeaa c oo oeoeo aeoo oacc ao Bacillus sp. 77-1 Rhodococcus sp. 78-5 (a. 10).

Taa oepc ooo pa eapoo co N-3E-ee-2-opo-oy aeaa peax aooa / aooa Cycpa Aeop oepc, % coo Bacillus sp.77-1 Rhodococcus sp.78-ac 3 / 6 / 3 / 6 / o p e- NH3 53 51 49 apoo co yao-1 0 0 - eao-2 0 0 - N-3E-ee-2--opo- yao-1 100 100 100 oyaea B peyae cceoa pacopa cycpao eee 8 aco p aax oepax 3 6 / ycaoeo, o, oo aoo ooo pa eapoo co a oaaaopa poxo c 50% oepce xapaepo cepeoceeoo poecca (a. 10).

O O O O NH+ +OH O NHo p eapa o eapoo co ) NH N Ac BuOAc BuOH + + N-3E-ee-2- N-3E-ee-2- -opo-oyaea -opo-oy p aae aooa N-3E-ee-2--opo-oyaeaa, peocaeoo poeccopo Apaxao .. (OX H PAH), yaoo-1 aee oaaaop e po cepeocex coc pacoppy cycpa a 100% (a. 10).

3.6.1. Be coa aaa a aoc oaaaopo cceoae coa aaa a aoc oaaaopo ooo ycao, o cooae apoaa ao eco aaa yeae aoc oaaaopo Rhodococcus sp. 78-5 Bacillus sp. 77-1 50% oepc ooo pa eapoo co ocaec ye 5 aca pea (pc. 7).

0 46 92 138 184 oepa aaa, o/ Pc.

7. acoc aoc ao Rhodococcus sp. 78-5 Bacillus sp. 77-1 o oepa aaa eceoo e aaa ( ) apoaa ao ( 78-5, 77-1).

78-5, 77-eee aoc, o oy, ocec e, o p cooa apoaa ao aa occ peaoy cec o epe pacope co, o coa ocoy oepa aaa peaoo cec eee ceo poecca aooa.

3.6.2. Be oepa pa a aoc oaaaopo yee oepa ooo pa eapoo co a copoc pea oaao, o c poco oepa pa o 9 / aoc oaaaopo o e eec, a p aee yee aeo aae (pc. 8).

77-78-0369 oepa, / Pc. 8.

acoc aoc oaaaopo a ocoe ao Rhodococcus sp. 78-5 Bacillus sp. 77-1 o oepa ooo pa eapoo co.

yee oooc oopaoo cooa oaaaopo p oepa ooo pa eapoo co 9,0 / Aoc, /(ac) Aoc, /(ac) 20,2 / apoaa ao eee 3 o oaao, o aoc oaaaopo aeo e eec.

3.6.3. oyee peapax oec poyo aooa ooo pa eapoo co cooae pao exooeco cxe paee paeecx po opaeco cpee (pc. 6) eee 3 o p aooe 27 ooo pa eapoo co oaaaopa a ocoe ao Bacillus sp. 77-1 Rhodococcus sp.78-5 ooo oy (S)-(+)- (R)-(-)-o c xoo 84-89% oeco coo 97-98% ee (a. 11).

O H H Rhodococcus sp. 78-5 + O CHCH2OH O O (R)-(-)-o O (S)-(+)-o O O p eapoo co + NHO NHH O H (R,S)-o p + eapoo co OH CHO CH2 O O Bacillus (S)-(+)-o sp. 77-1 (R)-(-)-o p eapoo co Taa Peya eecoo paee paeecoo ooo pa eapoo co oaaaopa a ocoe ao Bacillus sp.

77-1 Rhodococcus sp. 78-5 aooo eoyeoe oaaaop oe- oe- Ocaoa (R)-(-)- (S)-(+)- co cy- co - aoc, o o cpaa, o a acca, % xo, ee, % xo, ee, % Bacillus 30 27 3 80 - - 11,3 sp. 77-Rhodococ- 30 27 3 98 12 97 - cus sp.78-Oea oeco co pooac o caap ya pae:

(S)-(+)-oa []24= +22,50 (c=1,CH3OH); (R)-(-)-oa []24= - 220 (c=1,CH3OH).

3.7. cceoae aoc oaaaopo o yco pee xpae Ocyece oc cocoa eoo xpae oaaaopo.

caoeo, o p oao eepaype cpa oacca ye epe cy epe cpee o 20 % aoc, o e pe aoc opaoao aeoo oacc paec e eec.

yee xpaee cpo oacc p eepaype 10 0C eee 9 ece oaao, o oa epe o 70% aoc ye a 6 ece.

Taaacoc aoc aeoox opoo o eoc xpae a poecc Aoc, % 1 ec 3 eca 6 ece 9 ece Bacillus sp. po op-yaeaa 100 100 98 79-54 Ao op-yaeaa 100 100 95 Rhodococ- po -3-ocypaa 100 100 97 cus Aoo -3-ocypaa 100 99 99 sp.78-Aoo ooo pa 100 100 97 eapoo co po op-yaeaa 100 100 90 Bacillus sp.

Ao op-yaeaa 100 100 89 77-po -3-ocypaa 100 100 100 Aoo -3-ocypaa 100 100 97 Aoo ooo pa 100 99 99 eapoo co *-a 100% pa aoc ceeo aeoooo opoa B oe pe, ycaoeo, o xpaee oaaaopo e oeoex aeoo eo ec oee e e xpaee aopoeo cpo oacc. oaao, o aoc opaoax aeoo eo oce cceyex poecco caec eaeo ae epe 9 ece xpae. cee coca a Bacillus sp. 77-1, oop poeccax poa aoa op-yaeaa oce 9 ece xpae po aoc a 15-17% e cxoo.

Bo 1. B peyae cpa poopao ae epoa 3 ox eaoex aa: Bacillus sp. 77-1, Bacillus sp. 79-54 Rhodococcus sp. 78-5, cocoe aapoa aocee po paeecx op-yaeaa / -3-ocypaa.

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