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3.1.2. cceoae pacopa -3-ocypaa eece cceoa pacopa paeecoo -3ocypaa, oee p pax aax oepax cycpaa (3 5 /), oaa, o 5 ao (77-1, 77-7, 78-8, 77-36 78-5) po ey y cepeoceex pea (pacoppy ocp c oepce 47-58 % eaco o aao oepa cycpaa) (pc. 3). Ocae a (77-31,78-11 77-15) pacoppy cycpa ooc p x oepax -3-ocypa.

77-1 78-8 77-7 77-36 78-5 77-31 78-11 77-a (S)-(+)- -3-ocypa - 5 /; (R,S)--3-ocypa: - 3/ - 5/ Pc. 3.

oepc poa -3-ocypaa poopaa:

yco pea: pH= 7,0; t=30 0C; pe 5 .; aaa oepa cycpaa 5 /.

cooae aece cycpaa S-aoepa -3ocypaa ooo , o 4 aa (77-1, 78-8, 77-36 77-7) eee 7 aco poy o aoep a 80-90 %. B o e pe, oepc, % o oapyeo, o a 78-5 ooe e oeppye o aoep (pc. 3). o yaae a ae epeo S-a y ao 77-1, 78-8, 77-36 77-7, a ae a yopoae poa R-a y aa 78-5.

a H OH H HO + (S)-a COOEt COOH OH (R)-(-)--3-oc(S)-(+)-3-ocaca COOEt ypa coa (R,S)--3-ocypa OH OH a H H + COOEt (R)-a COOH (S)-(+)--3-oc- (R)-(-)-3-ocaca ypa coa oceee o oepeo cceoae oecx coc poyo ocaox cycpao, opayxc p pacopa paeecoo -3-ocypaa poopaa (a. 2).

Taa Bxo oeca coa poyo pacopa yco pea: pH= 7,0; t=30 0C; pe 5 .; aaa oepa cycpaa 5 /.

a -3-ocypa 3-ocaca coa Bxo, Oeca c- o- Bxo, Oeca o%* oa, % ee ypa %* coa, % ee ypa 77-1 100 99 R 100 95 S 77-7 100 80 R 82 71 S 77-36 100 88 R 83 72 S 78-5 100 100 S 100 96 R 78-8 100 96 R 86 92 S *- a 100% p eopeec oo xo poya p cooecye oepc paeecoo cycpaa.

p o o aeo, o aoee ce oec ae coee opayc p cooa S-ceoo aa 77-1 R-ceoo aa 78-5.

3.1.3. ea cceoae aoex coc ao ea aex epcex ao poopao o oco opooec, ooo-oxec paa, ooa oec a 77-1 79-54 poy Bacillus, a a 78-5 poy Rhodococcus.

B peyae cceoa aoeoc co H e pya oo eoea ycaoeo, o ce a apye eoax, e po eooc, aoc oceoc.

3.2. Papaoa oaaaopo C e papao oaaaopo a ocoe aex ao cceoac yco eoo poca oacc poopao e poao aoc.

3.2.1. oop aeo cpe paa poopao cceoae poca poopao a cpeax: CP-1epaa cpea c oo aco; CP-2 aapoa poa ; CP-3 aapoaoe cyco; oaao, o ao xo oacc ocaec p paa a CP-3 (a. 3).

Taa Be cocaa cpe a poc aoc poopao yco pea: pH= 7,0; t=30 0C; pe 5 .; aaa oepa cycpaa 5 /.

a Cycpa Bxo Aoc eo oacc*, % % CP-1 CP-2 CP-3 CP-1 CP-2 CP-Bacillus sp. 79-54 op-yaea 100 180 190 100 100 98,Rhodococcus sp.78-5 -3-ocypa 100 210 350 100 100 100,Bacillus sp. 77-1 op-yaea 100 170 230 100 74 86,-3-ocypa 100 170 230 100 76 98,a 100% p xo aoc 3-x cyoo oacc paeo a CP-caoeo, o aoc epeo y ao Bacillus sp. 79-54, Rhodococcus sp.78-5, e ac o cocaa cpe, a aoc epeo aa Bacillus sp. 77-1, paeoo a cpeax CP-2 CP-3 a 2-26% e, e p paa a CP-1. Oao, xo oacc a CP-3 2,3 paa peae oeco oacc, oyeo p apaa a CP-1. (a. 3).

Bcece oo CP-3 paa aoee eo cpeo oye cex 3 oaaaopo.

3.2.2. acoc aoc oacc o pee paa cceoae acoc aoc oacc o pee paa eee 3 cyo oaao, o y ao Rhodococcus sp. 78-5 Bacillus sp. 79-54 oa e eec, a y Bacillus sp. 77-1 aoc ac o opaca aa (a. 4).

Taa Aoc oacc acoc o pee paa yco pea: pH= 7,0; t=30 0C; pe 5 .; aaa oepa cycpaa 5 /.

a Cycpa Aoc, % 1 cy 2 cyo 3 cyo Bacillus sp. 79-54 op-yaea 100 98,2 102,Rhodococcus sp. 78-5 -3-ocypa 100 101,9 104, op-yaea 100 87,64 37,Bacillus sp. 77-1 -3-ocypa 100 83,3 29,Haoa aoc aaec y cyoo yyp aa Bacillus sp.

77-1, oa a y yxcyoo oacc oa aeo aae, o oppepye c aao ecoo copoopaoa. oce 3 cyo paa cop coca ooo 80% oacc, a aoc aae o 37-29%.

Ta opao, pe paa ao Bacillus sp. 79-54, Rhodococcus sp. 78-5 cocae poe cyo, a Bacillus sp. 77-1 o cy.

3.2.3. Be peopao aeoo a aoc eo ecoe copoopaoae Bacillus sp. 77-1 o oapyeo ae poecce pacopa xpae oacc p oao eepaype, o oppepoao c oepe ee aoc. caa aoc oacc a poeea oa peopa epea eo ye x opao aeoo. p o o oapyeo yeee poao aoc, o oe oycoeo eco a aae p opaoe eoop opaec pacope, a ae opacae poaeoc epa (a.5).

Taa cceoae peopao aeoo a aoc yco pea: pH= 7,0; t=30 0C; pe 5 .; aaa oepa cycpaa 5 /.

a Cycpa Aoc, % Cpa oacca Oeoee aeoo e 1 6 1 6 Bacillus sp. 77-1 op-yaea 100 66* 110 -3-ocypa 100 88 219 Rhodococcus sp.78-5 -3-ocypa 100 82 233 Bacillus sp. 79-54 op-yaea 100 58 158 a 100% pa aoc eopaoao oacc 1 e; * aoc - 3 e Opaoa aeoo cpo oacc cex aex ao, pea oe caa x aoc poeccax poa.Ta, aoc oeoex eo a 6 o pacopa aae a 4-9%, a aoc cpo oacc aae a 12-42% (a.5). Bcece oo cooae aeoox opoo aece aaaopo peoeee, e cooa cpo oacc.

3.3. cceoae yco a aoc peopaoao aeoo oacc p poe -3-ocypaa opyaeaa C e oca oax yco pacopa -3ocypaa op-yaeaa yeo e cooc cpe (pH), eepayp, aao oepa cycpaa copacope a aoc oaaaopo.

3.3.1. Be pH caoeo, o p paee paeecoo -3-ocypaa acaa aoc y aa Bacillus sp. 77-1 aaec p pH = 8.0, y Rhodococcus sp.78-5 p pH=7.5, a p paee op-yaeaa y Bacillus spp. 79-54 77-1 p pH=7,5 (pc. 4a).

3.3.2. Be eepayp B peyae cceoa acoc aoc oaaaopo o eepayp ycaoeo, o a Bacillus sp. 77-1 aoee ao poye -3-ocypa p eepaype 34 0C, a Rhodococcus sp.78- p 35 0C. p poe op-yaeaa a Bacillus sp. 77-1 aoee ae p eepaype 30 0C, a a Bacillus sp. 79-54 p 35 0C (pc.4).

a) ) 60 5,5 6 6,5 7 7,5 8 8,pH 20 25 30 35 40 eepaypa, C ) ) 50 45 40 35 30 25 20 15 10 5 0 0 1 2 3 4 5 6 7 8 9 10 0 10 20 30 40 oepa, / oepa aeoa, % Pc. 4. acoc aoc oaaaopo o a) pH, ) eepayp, ) aao oepa cycpaa, ) oepa aeoa peaoo cec; poeccax poa -3-ocypaa ( ) 77-1, 78- op-yaeaa ( ).

77-1, 79-3.3.3. acoc aoc oaaaopo o aao oepa cycpaa oaao, o a Bacillus sp. 77-1 aoee eo pacoppye -3-ocypa p aao oepa cycpaa 7 /, a Rhodococcus sp.78-5 p 6 /. p paee op-yaeaa a Bacillus sp. 77-1 eo paoae p aao oepa cycpaa 5 /, a Bacillus sp. 79-54 p 7 / (pc.4).

aoc,/((ac) ) aoc, /((ac) ) aoc,/((ac) ) aoc, /((ac) ) 3.3.4. cceoae copacope a po opyaeaa.

cceoae copacope a po op-yaeaa, oaao, o oepa aeoa, oeceaa acay aoc oaaaopa, cocae 30 % o oeo oea peaoo cec aa Bacillus sp. 77-1 35% Bacillus sp. 79-54 (pc. 4).

3.4. peapa ce oecx oepo -3-ocypaa, op-yaeaa, op-yaoa ocaco co.

oye peapax oec oec ax -3ocypaa, op-yaeaa, op-yaoa ocaco co papaoaa paa cxea eecoo paee paeecx cece poo po oe.

3.4.1. paa cxea eecoo paee paeecx cece -3-ocypaa op-yaeaa Ha ocoe poeex cceoa papaoaa cxea peapaoo oye oecx oepo ye aoceeoo poa op-yaeaa -3ocypaa oye oaaaopa cocoa 3 ca: 2 oe oox cycpao oyee oaaaopo eecoe paeee paeao poo oo ae, 3 ca eee oyex oepo peaoo cec (pc. 5).

1 ca ae ce: paae oacc epeepe (pH=7.0, t= 300C) eee 1-3 e acoc o aa, oeee oacc o cpe epye ( ) 2, opaoy e aeoo (aeo : oE acca, 3:1) oc ( ) 3, oeee oaaaopa o aeoa 4.

oye oaaaop oe xpac p 15 0C e eee 9 ece.

Ha 2 ca pooc eecoe paeee paeecx cece po 0,05M ocao yepe (pH=7,5-8,0; t= 30-350C; aaa oepa cycpaa 5-7 /; oepa copacope p paee op-yaeaa 30-35 % o. acoc o oaaaopa oP epa oopoo 30 /) eee 5 aco peaope ( ) 5 c ocey oeee peaoo cec o oaaaopa 6. oaaaop oe cooac oopo e eee 6 o pacopa e ep aoc.

Ha 3 ca cyae paee op-yaeaa (I) peaoa cec ocyae cpaop ( ) 7, e caaec NaCl (o ace), a ae poy pea cpapyc o po ( ) (p:

E 8 cec, 3:1). pa pa oeoaec Na2SO4 ae ooBP ec a ayyo-poopo capee ( ) 9. oyea cec oeX po paeec eoo oco xpoaopa ( ) a ooe ( ) 11 aoeo caee (e eca: aea, 3:1). pa coepae oec ae op-yao op-yaea copac E E 13 BP 12 , cooeceo aee e ooec a 14 15, a E E poy ocya 16 17.

oca aeo yep oacca oaaaop I. op-y c aea II. -3-oc ypa yypaa aeo oc oaaaop I. a) NaCl, II. a) NaCl Na2SO4 ) e ) NaOH, pH=eca:aea 3:1 pa ) eo pa eoa pa I.

II.

oa oa pa pa Na2SONa2SOpa pa a)HCl, 12 13 pH=) p 14 pa pa oa pa 17 (R)-3-ocaca coa (R)--3-ocypa (S)-op(R)-op-y(S)--3-ocypa (S)-3-ocaca coa yao aea 1-epeep; 2,4,6-epy; 3,8,19,22-oc c eao; 5-peaop; 7,18cpaop; 9,14,15,20,23-ayyopoope cape; 10,12,13,16,17, 21,24 oc;

11- ooa X, o p, e, c-cep oyx, eooce.

Pc. 5. paa cxea eecoo paee opyaeaa -3-ocypaa poo oo ae.

p paee poyo poa -3-ocypaa (II) pea oa cec ocyae 7, e caaec NaCl (o ace), oeaaec NaOH o pH=14, a ae ocao cycpa cpapyec eoo (eo: cec, 3:1). eoa pa oeoaec Na2SO4 E 22 ae eo ooec a 23. Oec a -3 BP E ocypa ocyae 24. Boa pa ocyae 18, e oc ec HCl o pH=2, a ae ocaca coa cpapyec.

E 19 pa pa oeoaec Na2SO4 ae ooec a BP E 20. Oec aa 3-ocaca coa ocyae 21.

3.4.2. oyee peapax oec (R)-(-)- ( aS)-(+)- oepo -3-ocypaa ocaco co B peyae eecoo paee 42 paeecoo -3ocypaa eee 6 o o papaoao cxee (pc. 5) oeoeoyx opaoa aeoo ea ao Rhodococcus sp.78-5 Bacillus sp. 77-oye (R)-(-)- (S)-(+)- oece oep -3-ocypaa 3-ocaco co xoo 80-90% (o eopeec oooo p ao oepc pa) oeco coo 95-100% ee (a. 6).

Taa Bxo oeca coa poyo poa -3-ocypaa a 6 o aa Bacillus sp. 77-1 Rhodococcus sp.78-5 oax ycox oaa- Oca- (R)-(-)--3- (S)-(+)- -3- (R)-(-)-3-oc- (S)-(+)-3- ocaop* oa ocypa ocypa aca coa aca coa a- - ee, - ee, xo, ee, % xo, ee, % oc, % xo, % xo, % Bacillus sp. 100 18,9 99 - - - - 17 77-Rhodococ- 90 - - 18,5 100 17,4 96 - cus sp.78-*-oepa: oaaaopa 30 /, cycpaa 7 /;

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