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o peyaa cxoec eo, o aoee oepa yco eeaa c ocep ocp copo cyacoe. Bcxoec ocpa cocaa: 26% - opo, 86% - oa c Poopo e cce appoa 90% - oa c Poopo cceo appoa; cxoec copo cyacoo - 12, 57 61% cooeceo (pcyo 6). peyeco peaaex o pace-oeopao ae ec o, o o ecx oy poo pec ceco xoce P. poe oo, copo cyacoe oocc oo paa cocoo coec aoooe c yeo aocpy aep.

a) epx co eeaa 100,90,80,70,60,50,40,30,20,10,0,180 330 360 Bpe, cy.

) co eeaa 100,90,80,70,60,50,40,30,20,10,0,180 330 360 Bpe, cy.

opo:eea e Poopa apa Heea+Poop Heea+Poop+ccea apa Pcyo 5 - oepaa e eepoyo p oce eeaa opeapao Poop oepaa, % oepaa, % oce ooo-aoo cec, cocoe ocpa copo cyacoo, oaa, o eea oce 360 cy. oc opeapao Poop e oaa aeoo eaoo ec a oopay oooaoy cec pa a poe ceo epoa x pa. Mopoooece xapaepc, ae a cxoec, oee peeo coa, coa paoco, opaca ce, a ope oe c Poopo e appoa oe c Poopo cceo appoa cyeceo e oac o opo (eape epoe) a poe ceo epoa.

o cape epa ocep Cyaca paa opo: eea e Poopa apa Heea+Poop Heea+Poop+ccea apa Pcyo 6 - Bcxoec pace oce ooc eeaa o e eepoyo opeapao Poop poyoc paocec oe c Poopo e cce appoa cocaa 85%, a oe c Poopo cceo appoa 98% o opo a eapeo epoee. Cypyee ece paceoeopao o aoee aeo x coe eeaa, oope xapaepoac oe cee ocaoo apeoc e eepoya o cpae c epx co (pcyo 7).

B poecce ooc eeaa c oo Poopa, ocooo pacooe eeaa epoea pee oeopao (ocpa cyaco pa) oeaoc yeee aoce ax epeo, a aaaa epoeaa, a ae epaa.

Aoc epa epe 540 cy. ooc eeaa o e eepoyo opeapao Poop ye appoa, ocooo pacooe eeaa epoea pee oeopao (ocpa cyaco pa) cocaa 68,4% epxe 52,3% e coe o aoc epa eapeoo epoea. cxo oo, o cceoa ox aopo (opo, Cyeao) oaa, o aoc epa ye pyx epeo opaae ypoe oopo ooeco Bcxoec, % opo aoc o, oo cea o o oe ooeco aoc cce.

a) epx co eeaa I II 0 30 60 90 120 150 180 210 240 270 300 330 360 390 420 450 480 510 Bpe, cy.

) co eeaa 90 I II 0 30 60 90 120 150 180 210 240 270 300 330 360 390 420 450 480 510 Bpe, cy.

opo:eea e Poopa apa Heea+Poop Heea+Poop+ccea apa -ooeoe ecee ooao: opa I aooca - oce ooo-aoo cec pa II Pcyo 7 - aa coepa e eepoyo eeae a poe ceo poecca ooc Oopaoe ecee opeapaa Poop oeceao ppoc eepopox aep a a opa. p o poypoae aep Rh. erythropolis, oce oopx ocyecc o xapaepoy x eoy a A, coca 60-86% oe ceoc eepopoo. B o pe a opoe acaa ceoc eepopox aep coCoepae e eepoyo, / o o Coepae e eepoyo, / caa ceo 6,0103 / o p pex oeax ceoc a poe ceo pee ypoa.

Mpoooece cceoa ceecoa o o, o aa exoo ooc eeaa poa oe aeey yee ceoc apox eoopapyax poopao ppyx aep. eco, o apoe eoopapyae poopa ppye aep aoee yce ape o e eoe pe ca ee yeaee oece, oea a o yeee ceoc pox eo (cao, 1968). Haac ppoc ceoc apox eoopapyax poopao ppyx aep ec ooe ceeco oo, o pocxoa oca epoo eeaa o e eepoyo.

Ta opao, peee opeapaa Poop, cce appoa oeopao (ocpa cyaco pa) ooe c coepae e eepoyo e oycaeoo ypo a 540 cy. a e, a epxe cox eeaa oeo ycao. Coepae e eepoyo epxe coe eeaa cocao 0,2 / o, e 0,3 / o epe 540 cy. (pcyo 7). Hopao oycoe coepae e eepoyo oe - 1 / o.

Papaoaa exoo ooe c coepae e eepoyo e oycaeoo ypo (1000 / o) a e, a epxe cox eeaa ycao a 540 cy. oyee peya oo cea peooee, o papaoaa exoo ooc eeaa o e eepoyo opeapao Poop oe c aoo oc ox ocao eeaox aapo ycox ceax ooo.

5 cooae aep Rh. erythropolis AC-1339 ooc ypox oxoo o opaecx oepo Tpyoyae ap ooeo ypox oxoo ec opaa, pecaea poo ao xecx peaeo, peex ype. B c c , p oope opaecx oao ypoo pacop eoxoo ya x ocooc c e a o x opyae cpe. e cceoa ac oea ocooc eoopx paxax peaeo (yypy paxa, paxa fito-R apoa EHV) oa M (M-Selpol-SL, M-Selpol-RX Mc) ooceo aep Rh. erythropolis AC-1339.

oe ocooc yaax paxax peaeo oa M ooceo aep Rh. erythropolis poee cepe o cpee Maaa c oaee aece aopa poca poeoo aoaa. B aece eceoo coa yepoa ep oa cooecy ypoo peae: M oece 0,3, 0,5 1,0% acc.; paxa peae oece 0,5 1,0 % acc. ypoae poo aaox oax a epocapoao aae p eepaype 30C acoe pae 100 -1 eee 8 cy, eee 16 cy. o coepac epocae p 30C. O cee oepaa paxaa cy o yee oeca paxaa, a ae oceo o ppocy ceoc aep. O cee oepaa M cy o ee yeo oc yypao oc, a ae oceo o ppocy ceoc aep ee pH cpe.

Ececeoe paoee paxax peaeo M aopeo poopo e peaa cpee 3,0% a 10 cy. ooceo epoaaoo coepa. cxo oyex peyao, paccapaee paxae peae o ocooc oo pacoo ceye ope: paxa peae apoa EHV > yypy paxa > paxa fito-R (aa 3). Peya oo p aax oepax M 0,3 0,5% acc. oaa, o o ocooc cceoae oa M pacoaac oceoaeoc: M-Selpol-RX > M-c > M-Selpol-SL (aa 4).

Taa 3 - Cee oepaa paxax peaeo aep Rh. erythropolis AC-1339 o epao cpee Cee oepaa, % Haaa oepa Haaa oepa paxa peae 0,5% 1,0% 4 cy. 8 cy. 10 cy. 4 cy. 8 cy. 10 cy.

apoa EHV 64,0 83,6 88,1 65 82,9 85,yypy paxa 71,1 90,1 97,0 69 87,4 90,paxa fito-R 75,4 93,2 98,5 72 89,1 92,Taa 4 - Cee oepaa pax oa M aep Rh. erythropolis AC-1339 o epao cpee Cee oepaa, % Haaa o- Haaa o- Haaa oMoa epa 0,3% epa 0,5% epa 1,0% M 4 8 10 4 8 10 4 8 cy. cy. cy. cy. cy. cy. cy. cy. cy.

M-Selpol-SL 63,0 96,8 99,3 69,0 88,3 94,0 63,6 87,5 90,(C=258) M-c 60,0 92,0 99,0 64,1 87,0 92,0 57,1 84,0 88,(C=424) M-Selpol-RX 53,0 84,6 98,1 58,0 79,4 88,6 40,0 73,0 82,(C=788) Peya ppoca ceoc aep Rh. erythropolis a cceyex paxax peaeax oax M oaa, o aa ceoc poooo o cex oax a cxoo paaac oceeo yee x oeca a a opa oceye ce oy cepea a 10 cy. Bece c e, ceoc poooo a pao acea o a coyex paxax peaeo oa M. B oax c paxa peaea aoa ceoc aep a poe ceo cepea aaac apae c ep a poe ceo cepea aaac apae c paxao fito-R, a aea c apoo EHV.

B oax c oa M aoa ceoc aep a poe ceo cepea aaac apae c M-Selpol-SL, a aea apae c M-Selpol-RX. Aa ppoca ceoc poooo oceo oepa peee e pacooe oa M paxax peaeo o ocooc. Coocaee xapaepa ee ocooc oa M co cee x oepa oaa, o oepaa M aep Rh. erythropolis AC-1339 axoc opao acoc o x cee oepa.

oyee ae o ocooc paxax peaeo oa M eoxoo ya p oope opaecx oao ypoo pacop c e a opyae cpe.

poeeo oppoae aeaeco oe, ocae e eepayp (o 10 o 40C), pH (o 5 o 9), oepa M (o 0,5 o 1,5% acc) xopcoo ap (o 1 o 5% acc) a oaccy aep Rhodococcus erythropolis AC-1339 c e oe eo aoc ecpypoa M. B peyae poeex cepeo paceo ypaee pepecc ee cey : y=0,049 + 0,324x1 + 0,093x2 + 0,002x3 + 0,064x4, e x1 oepa M, %; x2 oepa xopcoo ap, %; x3 eepaypa, C; x4 - pH. Ta opao, aoee e a ppoc oacc aep Rh. erythropolis oaae oepa M. p o p yee eepayp, pH oepa M pocxo ppoc oacc, a p yee oepa xopcoo ap cee oacc aep Rh. erythropolis.

6 poee ca cocoa a ex ape c oo opeapaa Poop, oex oao oeopao o poeeo poeoe cae cocoa a ex ape c oo opeapaa Poop, oex oao (op aooc) oeopao (copo cyacoo ocpa ocpoo) opae paoe aeo cooo ae o 2 a eppop OAO Hooyc eeepepaaa ao (HH) Pecy aopoca. Cyce opeapaa Poop oya a poeo ycaoe aoeecoo oxoaa, . aoeec Pecy aopoca. Pacxo o cyce opeapaa Poop coca 1,0 - 1,3 /2 acoc o cee ape. opeapa aoc ye oea aocep c a co. epe 40 cy. a ce eppop ooeo oc ooa poopao: op pacea 8 - 10 /2 aooc 1 - 2 /2 ( cyxo e). Oopeeo poo pxee. Ha eppop oa 90 2 poe ace cec pa: ocep epa, x coooe 1:1.

Hecop a opo cpo ca eaope eeeoc poopao ooe yco (o o pe opao acy epo a poe oceyx 20 - 30 cy.) o yaco xapaepoac oee co ppoco ceoc eepopox poopao (a 4-6 opa) o cpae c opo yaco (a 1-opa). p aae cxoec pa a 40 cy. oce ocea oeeo oee poco paec o ce oepxoc ooo yaca. p o a 4 ec. a ocya coa cee oc o ooo yaca o e eepoyo cpee 83,1% co 0 - 10 c 64,2% 10 - 20 c, o a 72,7 58,4% oe cee oc o o e eepoyo a opoo yace.

poeee pao ee peya oepe ao o ooopy HT/y/3 1/2/4964/00/OOC OAO HHǻ o 19.05.2000. Ta opao, coco peya apeo eepoya o c oo opeapaa Poop, oex oao (op aooc) oeopao (ocpa ocpoo copo cyacoo) oaa coy eoc ycox aopocaa oe peoeoac pooo epe p a ex ape o aecx ycox Pecy aopoca.

BBO 1. Bepe ycaoea cocooc aep Rhodococcus erythropolis BM AC1339, xc ocoo eeoceo opeapaa Poop, poypoa oyaop yeoopoo e p poce a aoe, ecaeae e.

2. oaaa cypya po poyo oce epoea cae a poc eeocy aoc aep Rhodococcus erythropolis BM AC1339, xc ocoo eeoceo opeapaa Poop, a o cpee, a oe. p o ecee BM oece 0,002% acc. ooe ecpoa poecc oepaa e eepoyo aep Rhodococcus erythropolis BM AC1339 a 20 - 40% a 1 - 3 cy.

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