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Ha paax pyoc HA EEHA EHHAEBHA Papaoa exoo ooc ex ypox oxoo 03.00.23 oexoo Aopeepa ccepa a cocae yeo cee aaa execx ay a - 2002 Paoa oea a aepe ox exoo poooecx pooc coo ocyapceoo eoo execoo yepcea HAH POBOTE:

aapoa oop execx ay, poeccop ye ayoa OAHE OOHEHT:

oop ooecx ay, poeccop yo pa Eeea oop execx ay, cap ay Mao copy Ha Cyao Beya opaa: cy eexepepao, .a aa cococ 22 op 2002 oa aco a acea ccepaooo coea 212.289.06 co ocyapceo eo execo yepcee o apecy: 450062, aopoca, . a, y. ocoao, 1.

C ccepae oo oaoc oee coo ocyapceoo eoo execoo yepcea.

O a aopeepa yx epax, aepee epoo ea, poc apa o apecy: 450062, aopoca, . a, y. ocoao, 1, eoy cepeap.

Aopeepa paoca 2002 oa.

e cepeap ccepaooo coea, oop execx ay, poeccop H.A. Caoo 3 OA XAPATEPCTA PAOT Ayaoc poe. Pacpocpae coa ape o ooo e eepoya c pep eeo, eeepepao, pacopa e poyo e epepao. oaa oy oo, e eepoy oy po ooce ooec aacpoa a aex oax. B peoax c pao eeoae poeoc e ape oac oce ypoe oxo.

oaa opyay cpey, e, eepoy ypoe xpeae opaeo ecy a ce e ooeco e, po apye ececex ooecx xecx poecco. Oae occe, oepac ocecoy ec eepoyo, coco ayypoa x cox ax. eoopo oy ae o e e epeaac opa eoea (apep, aepoee oece ooe e).

Haoy oacoc occe peca ypoe ee oxo, oope aaac xpac, a pao, ex aapax. ece coco oc ya ex ypox oxoo (ocoeo ox ooe eeao) - exaece, xece ece e cea e, a ae aco ooec eeoac. B c c epce cocoo oc ex ypox oxoo o e, eepoyo xpeaeo ec cooae oeca ep, c oae ee oexooecoo eoa.

e pao papaoa exoo ooc ex (oe ooe) ypox oxoo ye cooa opeapaa Poop, ooao oeopao.

B cooec c ocaeo e peac ceye aa:

- oc poyeo oyaopo yeoopoo e oeox eeocx yyp;

- oea pee poyo oce epoea cae aece cyopa poca eeoce aoc aep Rhodococcus erythropolis BM AC-1339;

- oop eopao pacex oxoo (cooa, oca oox pa, o pea eyxa) eca poecca ooc eeaa (oe ooe) o e eepoyo opeapao Poop;

- papaoa exoo ooc eeaa (oe ooe) o e eepoyo opeapao Poop ye appoa, ocooo pacooe eeaa epoea, pee oeopao;

- oea ocooc eoopx ypox oao (paxax peaeo M) ooceo aep Rhodococcus erythropolis BM AC-1339;

- oppoae aeaeco oe, ocae e eepayp (o 10 o 40C), pH (o 5 o 9), oepa M (o 0,5 o 1,5% acc) xopcoo ap (o 1 o 5% acc) a oaccy aa Rhodococcus erythropolis BM AC-1339 c e oe eo aoc ecpypoa M.

Haya oa pao. Bepe ycaoea cocooc yyp Rhodococcus erythropolis BM AC1339 poypoa oyaop yeoopoo e.

oaaa cypya po poyo oce epoea cae a poc eeocy aoc aep Rhodococcus erythropolis BM AC1339 a o cpee, a oe.

oaaa oooc cooa eoopx pacex oxoo (oca oox pa, cooa, o pea eyxa) aece eopao eca poecca ooc eeaa (oe ooe aoaoee) o e eepoyo opeapao Poop.

Papaoaa exoo ooc eeaa (oe ooe aoaoee) o e eepoyo opeapao Poop ye appoa, ocooo pacooe eeaa epoea, cooa oeopao.

Bepe poeea oea ocooc eoopx oepx ypox oao: paxax peaeo (yypy paxa, paxa fito-R apoa EHV) M (M-Selpol-SL, M-Selpol-RX M-c) ooceo aep Rhodococcus erythropolis BM AC-1339.

poeeo oppoae aeaeco oe, ocae e eepayp (o 10 o 40C), pH (o 5 o 9), oepa M (o 0,5 o 1,5% acc) xopcoo ap (o 1 o 5% acc) a oaccy aep Rhodococcus erythropolis BM AC-1339 c e oe eo aoc ecpypoa M.

paeca aoc. Peya poeex cceoa c ocoo papao exoo ooc eeao (oe ooe aoaoee) ye cooa opeapaa Poop, ooao, oeopao, cce appoa, ocooo pacooe eeaa epoea. Papaoaa exoo oe cooaa p poee peyaox epop eeaooae eeaoepepaaae opacx poeoc.

poee poee ca o a ex ape opae paoe aeo cooo ae o 2 a eppop OAO Hooyc eeepepaaa ao (aopoca) c oo opeapaa Poop oeopao.

Apoa pao. Ocoe ooe ccepaoo pao pecae a ayo oepe o poocy pee aepao xecx peaeo Xece pea, peae poecc aooao x PEATB-1999, 1999; a V Meyapoo oepe o eca eexecx poecco Heex - 99, Heac, 1999; a 2-o Meyapoo coye Haya exoo yeoopox cce, a, 2000; a 2-o Bcepoccco ayopaeco oepe Oxo 2000, a, 2000; a 55- eo eyoco cyeeco ayo oepe P e aa e .M.ya HET A 2001, Moca, 2001; a opecce Heepoeo Pocc Heeepepaoa eex, a, 2001; a oe-ceape Xeca oo, a, 2001; a 50, 51, 52- ayoexecx oepex cyeo, acpao oox yex HT, a, 2000 2002 .

ya. o ee ccepa oyoao 11 pao, o ce oye 1 ae P.

Cpyypa oe ccepa. ccepa oea a 185 cpaax aocoo eca, co ee, oopa epayp, eo, cepeao ac, ocye peyao cceoa, oo, cca epayp poe, ae 39 a, 18 pcyo, 2 oopa. Cco epayp ae 180 epaypx coo, o ce ocpax 24.

Aaec oop epayp. B oope poee aa e, eepoyo ypox oao a oy oy cpey. Paccope poopa, opeapa, a ae coco peya, peaaee oc o o o e eepoyo. Paccope coco eca ooc o o e eepoyo ye pee epax opaecx eopao, ye oopa cea oeopao. B oope ae pee cee o poopaax poyeax oAB, o ax oAB, o opacx x pee, acoc o ayaoc cooa oAB p a ape opyae cpe e eepoya.

Maepa eo cceoa. B cceoax cooac eea (oe ooe), oopa aoaoe o OAO aeex (coepae e eepoyo eeae cocao 11,0% acc.). B cepeax ae pec epoe peo eco.

p poee cepeo pe poooece eo cceoa: opeeee oeca eo ae eoo oxa; opeeee poo oacc o epao cpee c oo epax po; opeeee oeca ppyx aep apox eoopapyax poopao oep eoa ye cea a cooecye cpe (cpea Bopacoo ecoa).

Opeeee ooceco aoc BM poyo x paoe, ooceco aoc poyo poooeco epaa yeoopoo e poo a popocax ce oca, yypy (p pexe ( MP 33 1 CX x F 22 C) X 1866/82 CB), oa eoo (Metilotus albus), ocpa ocpoo (Bromus squarrosus), copo cyacoo (Sorghum sudanense), ep opoo (Medicago sativa), a eoo (Lupinus albus) capea opooo (Onobrychis sativa).

aopaopo-aaece cceoa opao oopyo poo cooec c oep ooee eoa.

eoopo e oe o cpee opee cepoooepec aoxpoaopaec eoa.

oeco paxaa opee poepec eoo c oo pacopa ea. cooc M ecpy oea o ee yeo oc c oo oepa.

poeea caceca opaoa peyao.

PETAT CCEOBAH X OCEHE 1 oc poyeo oyaopo yeoopoo e oeox eeocx yyp eco, o oocyoc oxo pacopx opaecx apee a e eepoyo oo ye a ce cooa AB, o ecex eco ape, o poypoax poopaa in situ. B c c eca aya ec oc poopaopoyeo oAB. Ha a ocaea aaa poye oAB cpe oeox eeocx yyp Bacillus subtilis 1742D, Pseudomonas putida 1301 Rhodococcus erythropolis AC1339.

B peyae poeex cceoa o eo, o oo p paa aa Rh. erythropolis AC1339 a aoe, ecaeae e aaoc cee oepxocoo ae yypao oc poee ypye aoc.

p o oa coo yepoa cea oAB oaaac e: p poce aa a o cycpae oepxocoe aee caoc o 43,1 H/, ypya aoc o ooe epocy cocaa 59%, o pe a p poce a ecaeae ae oepxocoo ae ypye aoc coca 50,9 H/ 40,0%, a a aoe - 65,5 H/ 11,0% cooeceo.

ypya aoc yypao oc (cyepaaa) p ypoa aa Rh. erythropolis a epao cpee c e (1% acc.) acea o pee oopa (pcyo 1). Bea E24 yypao oc ocaa acya (59,0%) a 4 cy. p o oAB c aco eeo, a a ypyy aoc cee oepxocoo ae poa e oo yypaa oc aep Rh. erythropolis, o ee cyepaa.

B poecce ypoa oepxocoe aee yypao oc e cyepaaa peo oaoc ye a 1-e cy. ocao ya a 3- cy. ae aac epo eeoc (pcyo 1).

p opeee ypye cocooc yypao oc e cyepaaa o oeeo ee opae a yc, opaoao yypao oc (cyepaao) epoco. Ta, eee 8 cy. aoee ycoo yce ac oa/epoc, o pe a yc epoc/oa papyaac a ecoo y. Too a 9 10 cy.

pocxoo opaoae ycoo yc epoc/oa e aeoo opaoa opao yc.

aece aa opayex oAB c oo ecoo eoa, ocoaoo a cooa pace eeooo oyoo, oo , o a 3, 4, 5 6 cy. cpee, epoo, peoaa aooae AB, a a 9 10 cy. aooae AB.

E24, % N, /, H/ 2 4 6 8 10 Bpe, cy.

1 - ypya aoc (E24) cyepaaa, 2 - ypya aoc (E24) yypao oc, 3 oepxocoe aee () yypao oc, 4 - oeco eo (N), 5 oepxocoe aee () cyepaaa Pcyo 1 - eee apaepo yypao oc e cyepaaa p poce aa Rh. erythropolis AC1339 a epao cpee c e (1% acc.) yypaa oc aep Rh. erythropolis poa cocooc ypoa pae yeoopoe cycpa. p o aoy ypyy aoc yypaa oc Rh. erythropolis poa o ooe e (64%), ae epocy (59%) ecaeay (56%).

yee eepayp pooeoc popea a ypyy cocooc cyepaaa yypao oc oaao, o cepyeoe ao Rh. erythropolis oAB epocao: ypya cocooc oAB p epa eee 25 p eepaype 100C caac e oee, e a 20% (c 32% p 0 o 26% p 25 ).

Ha ocoe aa Rh. erythropolis AC1339 coa e eeoc opeapa Poop (aapoa, Copoa). eco, o ypoae e eepoyo cpee ecpye x oepaa. oo cpey, apey e eepoya, oc AB, poopa, poypye AB in situ. ooy cocooc aoo aa Rh. erythropolis AC1339 ae a yeoopo cycpa eo cocooc poypoa aco eeoe yaop yeoopoo e c ecoe peyeco opeapaa Poop. coca aa Rh. erythropolis AC1339 oo ocy cox peyao oc cpe o e eepoyo e ece ax-o ooex eeyaopo.

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