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Taa Tpacopa AA aocepe aoa oyxa oacco Pichia sp.80-11, paeo aapox ycox co oepc, Bpe, poy Bxo, Oeca pea % % coa, % ee Boyx 100 48 S-O 98 Ao 100 50 S-O 98 oee oo, o ycaoeo, o aa oacca oeceae ce S-O paec c e e oaae oyo aocepe, oac o oaaee oacc, paeo apox ycox (a. 5 6). o ooe peoaa, o aapoo paex eax Pichia sp.80-11 ocycye aa occaoea ccea R-a.

Oeca coa, % ee 6.3. Boccaoee AA oacco Pichia sp.80-11, paeo a aoe Boccaoee AA aapoo paeo oacco Pichia sp.80-11 ooe oy S-O cooo aeca, o aae ooo oo pee, c c e a pepa ooea oa yy ceeoc apoo pacopa eopa oacco, paeo pcyc oyxa, ye oopa yco, oeceax paeoe ycee aa apopeyao cce S-a o poecc.

a cocooc Pichia sp. 80-11 ocyec cpooe poee, o peooeo, o, a cyae eapcx poe, occaoe AA ocoy po oe pa S-cea aooepoeaa (Stewart, 2000). caoeo, o aooepoeaa yopye y aa Pichia sp.80-11 apox ycox, ocoy p poce a cpee c co coepae caxapo (oee 1 %) o poopa ocyece poee c pee aoee cpee aoa, oop ae accpyec poecce xa (e pp).

B peyae accpoa a cpeax c co oepa aoa (3 - 7 %) a oyea oacca, oaaa apopeyao aoc (caape yco), 3,7 paa peae aoc pexcyoo oacc, paeo a cpee c caxapoo. p o aaoc eooe yeee oeco co poya o 92 % ee.

7. cceoae oooc oopoo cooa oacc occaoe AA C e eca poecco aoceeoo occaoe AA ae poopaa a oeea oooc oopaoo cooa x oacc oye oec ax poyo.

B peyae cceoa pao oacc poopao caapx ycox eee ecox o o oapyeo, o ee apopeyaa aoc caec oee e a 50 % cyae Pichia sp.80-11 oce 3 a, a cyae aa Metschnikowia sp. 84-13 oce 4 a pacopa (pc. 6). B cyae aa Pichia sp.80-11 ee eco yeee aoc oacc oce 1 a.

Oeca coa S-O, cepyeoo ao Pichia sp.80-11, ecoo yeaec oce 1 a, o aae aa oce 2 a, o e pe oeca coa R-O e eac eee epx 4 o, aae opaca 7 y.

oye ae, yaae a o, o yeee aoc oacc aa Pichia sp.80-11, aaeoe o opo e pacopa, oppepyee c opacae oeco co (S)-O, apy e cao c poecco occaoe AA. oo e oapyaec oce peapeoo epa oacc caapx ycox pea, o e ece AA (pc. 7).

100 0 1 2 3 4 5 6 Hoep a oeca coa (R)-O (Metschnikowia sp. 84-13) oeca coa (S)-O (Pichia sp.80-11) aoc oacc Metschnikowia sp. 84-aoc oacc Pichia sp. 80-eee aoc oacc oeco co poyo Pc. 6.

p eco pacopa AA 100 Oeca coa (S)-O aoc oacc 0 0 4 24 48 Bpe, acoc ceex coc aoc oacc Pc. 7.

o pee peya Pichia sp.80- o ooe paccapa peya oacc a oxo oe copoc ceeoc occaoe AA ao Pichia sp.8011. caoeo, o ooe e o oepa oe ocy eee 4 .

Oeca coa, %ee Oocea aoc, % aoc, % Oocea Oeca coa, % poe oo, pecaee a pc. 7 ae ceecy o coo caoc apopeyao aoc ceeoc oacc aa Pichia sp.80-11 ocyce AA eee 72 (pc. 7). o ooe peoaa, o aaeoe a pcye 6 yxyee aaecx aoceex coc oacc p cooa eee 5 o pacopa AA (cyapoe pe ooo 20 ), copee ceo, cao c coee eox aaco occaoex oepeo NAD(P)H, coyxc poecce occaoe cycpax oecax.

8. oc oex occaoee peeepa opaoao oacc B cyae pee eoeox aaaopo aoee ocy cocoo peeepa occaoex op oepeo ec cooae oex coee, ocxc o ece NAD(P)+-acx epoea, exc eax (Matsuda, 2002). B c c a pepa oa peeepa aoc opaoao oacc ye ee ypoa pcyc oex occaoee (aoa, aaa caxapo).

B peyae cceoa o ycaoeo, o oo cceyex coee e occaaao apopeyay aoc oacc aa Metschnikowia sp. 84-13. Bece c e o oapyeo, o epae coeo oacc aa Pichia sp.80-11 eee 4 0, 05 % pacope aoa e oo occaaao cocooc eo pacoppoa AA, o ycao x aoc o cpae c cxoo o 1,6 paa (pc. 8).

1 2 3 4 Hoep a cea oacca opaoaa oacca peeeppoaa oacca (pacopa e aoa) peeeppoaa oacca (pacopa c aoo) Tpacopa AA peeeppoao oacco Pichia sp.80-Pc. 8.

aoc, % Oocea Oao aa peeeppoaa oacca coxpaa co oey aoc eee ooo a, oce eo oa aaa aa.

o ycaoeo, o caa aoc oacc peoeee o ao eocpeceo peaoy cec oopeeo c AA. B eo pcyc peeeppoaa oacca coxpaa aoc, o pae epe, eee 5 o pacopa (pc. 8).

B oax co cee oacco o oaao, o ao oo o o oepa 10 / e oae apopeyao aoc (pc. 9).

cooae oee cox oepa cpa po ee ce.

0 5 10 25 50 ao, / Be aoa a pacopa AA Pc. 9. Pichia sp.80- oaao, o oacca, peeppoaa a cocoo oeceae ce S-O e eee 94 %ee caapx ycox.

9. Papaoa ooaoo oaaaopa a ocoe eo Pichia sp. 80-Boooc eoo cooa oacc poe Pichia sp. 80-11 oycoa eecoopaoc papao ooaoo oaaaopa, peee oopoo aeo ypoae poecc eo pea eee poya. B c c a ocyecea oa eo ee oapa e. B peyae cceoa pao aoo oaaaopa a ycaoea eo coa aoc, caoc cocooc peeeppoac aoo.

10. Papaoa peapax eoo oye S- R-aoepo O C e oye peapax oec S R-aoepo O a papaoaa cxea oye x coee c oo ooax eo Pichia sp. 80-11 coox eo Metschnikowia sp. 84-13.

p peaa o cxe c peee aopaopoo oopyoa a ocoe oacc Pichia sp. 80-11 oye oapa oaaaop, oo a o pacopa oopax oo aa aoc, % Oocea ycox (pH = 7,5; cycpa 5 /; eepaypa pea 32 0C, pe pea 4 ) oy 4,5 S-O e eee 98% ee.

c x A TEME A A yep NaCl EEX p yep po EEoacca Na2SOopaoa AA yep ao Eyep yep EEpNa2SOEp I poaaaop I a peeepa oaaaop P _ (S)-O I _ (R)-O paa cxea cea S-O ooa oaaPc. 12.

aopo Metscnikowia sp. 84-Pichia sp. 80 11 R-O oaaaopo I - oaaaop Pichia sp. 80 II - oaaaop Metscnikowia sp. 84-A-apa, A-capa, A-epcya ao, TEME-NNNNepaeea, c cep oyx, x xooa oa, ea oa 1- epeep, -epya, E1-7 - eoc, X-xoo, - payop, p1,p2 - p, - cpaop, P - poopo-eo cape.

C oo eapeex eo poe Metschnikowia sp. 84-13 aex oax ycox pacopa (pH = 6,5; cycpa 5 /; oacca 8,5 (ac)/; apoe yco, eepaypa pea 32 0C, pe pea 40 ), ocyece ce (R)-O oeco coo 98 % ee.

11. cooae S-aoepa O cee epooo aceox Opa S-O epea OX H PAH, e aopaop ooeypx opeyopo aceox (aey aopaope - .x..

ypao..) ocyece aoceee ce epooo aceox peee cecoxocex pace.

OAc OAc 2S-peaea - ooo epoo 2S-pe-10E-eaea - ooo epoo ooo y (Drosophila mulleri) ecceco yx (Mayetiola destructor) OH O OC2HS--3-ocypa O O O O oayp-II oayp-I ooe apeaooo epooa epooo oa (Rhyzopertha dominica) oye 2S-peaea (ee ~ 97%) ooo epoo ooo y (Drosophila mulleri, Drosophila buscki), 2S-pe-10E-eaea - ooo epoo ecceco yx (Mayetiola destructor), aocx oo yep ocea ox pox e S-1-eyoe p 2-e- 2,4ee-2E-eox co, ece o aae oayp-I (I) oayp-II (II) cooeceo, oope xo coca apeaooo epooa epooo oa (Rhyzopertha dominica) - pee epox aaco.

BBO 1. B peyae cpa ppox coo ee epoa poopa Pichia sp. 80-11, Candida sp. 81-12 Metschnikowia sp.

84-13, oaae coo apopeyao aoc.

2. Ha ocoe ao Pichia sp. 80-11 Metschnikowia sp. 84-13 papaoa aoceee oaaaop, cocoe ocyec aoceeoe occaoee aeoaeaa c oece xoo S- Raoepo -3-ocypaa coo oeco co.

3. caoeo e apaepo poca oacc (pee ypoa, apox aapox yco, pax coo yepoa) pacopa a aoc ceeoc oaaaopo.

4. Hae yco, ooe oeceo oya S--3ocypa c coo e eee 98 %ee. oaao, o aoep opayec p ycox pea: eepaypa 32 0C; pH yepa 7,5; oepa cycpaa - 5 /; oepa eo 8,5 (ac)/ p cooa oacc Pichia sp. 80-11, paeo aapox ycox, eee 50 oacc, oyeo apox ycox, eee 4 .

5. Be yco, ooe oeceo oya R--3ocypa c coo e eee 98 %ee. oaao, o aoep opayec p cooa aa Metschnikowia sp. 84-13 oapeco ae poca apaepax pea: eepaypa 32 C; pH yepa 6,5; oepa cycpaa 5 /; oepa eo 8,5 (ac)/, pe pea 40 aco.

6. oaaa oooc oopaoo pee coax oaaaopo eee eoo pee, a ae oooc peeepa oaaaopa a ocoe aa Pichia sp. 80-11 eoocy oe occaoee aoo.

7. ye oa eo aa Pichia sp. 80-11 oapa e coa oaaaop oopaoo cooa aoceeoo occaoe aeoaeaa S--3-ocypa.

8. Ha ocoe poeex cceoa ayo oocoaa paa exooeca cxea oye S--3-ocypaa R--3ocypaa - coo ooeypx opeyopo c cooae papaoax oaaaopo a ocoe ao Pichia sp. 80-11 Metschnikowia sp. 84-13.

Cco pao, oyoax o ee ccepa 1. H.. eyxoa, A.P. aeoa, .B. paoa, B.B. op. Cepeoceeoe occaoee aeoaeaa poopaa. apc xec ypa1998,.5,3.-C. 42-45.

2. N.I. Petukhova, A.D.Zinnurova, A.R. Davletova, V.V. Zorin. Stereoselective Reduction of Ketones with Resting Cells of New Strains of Microorganisms//8th Meeting on Stereochemistry, Praga, 1998, p. 24.

3. aeoa A.P. aep . op B.B.Cepeocee ce coo ooec ax eec ooccaoee apocoepax coee.

//aoo ayo-pa. o. "oexoo "epa"", Ca eepyp, 28-30.cep 1999 ., c 50-4. aeoa A.P., eyxoa H..,op B.B. Hoe a oye Rocypaa ye cepeoceeoo occaoe. II e. o. oox yex "Ayae ee opaeco cee a opoe oo p", . Ca eepyp, 28-30 cep 99 , c. 42.

5. aeoa A.P., eyxoa H.., op B.B. cceoae e occaoe aeoaeaa oe poopaa.// poe eeaooo oeca Pocc, // Te. oao e. ayo-execo oepe, oceo 50-e HT, a, 13-15 a, 1998, c 46.

6. aeoa A.P., paoa .B., Copooyoa E.B., eyxoa H.. Mpoooecoe occaoee eopyeoa.// Maepa 49- ayo-execo oepe cyeo, acpao oox yex, ocea 50-e HT, 1998, c 7. aeoa A.P., eyxoa H.., Bacea B.B.,op B.B. oc ao, cocox opacopa paeecx oeaox oo. "Xece pea, peae poecc aooao x"// Te. o. XII ey. o.

o poocy pee xecx peaeo "Pea-99", Moca, 7-8 cep 1999, c. 69.

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