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cceoae oeco co poyo occaoe oaao, o 3,3-e-2-oco-ypoao occaaaec c o aoceeoc a ypoe 10 %ee, o e pe oee coe ae oeco co poyo oye cyae occaoe aeoo (ooo 70 %ee).

Ta opao, oyee peya oaa oooc cooa yaax ao aoceeoo occaoe apocoepax coee.

4. oc eoo oae oox pea ooccaoe AA Coaco copee peoa oeca coa coo ooeypx opeyopo oa e eee 95 % ee. B c c ocyece oc cocoa oae aoc eaeo epea, aapyeo opaoae eeeoo aoepa poeccax occaoe AA ao Pichia sp. 80-11 (S-a) Metschnikowia sp. 84-13 (R-a), c oo ecx xecx opo apopeya, a ae epeco peopaoo oacc.

4.1 Be xecx opo o cceoao ece a aoc poopao aooo cpa, paeo oaeo aoc aaepoea eapcx poe, pocoo aa, pyeo aooepoeay x poopao, apooo co ceapaa ap, oax aoc cea px co, ae yacye occaoe AA y eapcx poe (Faber, 1997; Nakamura, 1991).

cceoae oecx coc poyo pacopa AA oaao, o op a eoopoe eee ceex coc oox ao, oao poy peyeo co oce cyae oy e yaoc (a. 4).

Taa Be opo a poecc oaaecoo occaoe AA caapx ycox Pichia sp. 80-11 Metschnikowia sp. 84-op oe- Bxo, Oeca oe- B- Oeca pa % coa pa xo, % coa - S-O, % - R-O, % opa, / opa, / e opa 0 99 88 0 76 72,Ao cp 1 28,9 55 0,25 50 85,poc a 0,25 42,0 99 0,05 50 Ceapa ap 1 100 84,7 - - apooa - 1 100 57 - - - coa - e cceoac Bcoa oeca coa O a oaaa oo aa Pichia sp. 80-11 c cooae pocoo aa. Oao, peee aoo oxoa yee cepeoceeoc occaoe AA eca poeao, ocoy pea aeo aeec, aca xo poya e peae 42 %.

4.2 Teepaypa peopaoa oacc Pichia sp. 80-p epocapoa oacc Pichia sp. 80-11 p 52 0C paa caoc epeo e a oapyea. Haaeoe poecce apea cee aoc e oaae po paeoo a oecy coy opayeoc S-O, o ceecye o eceeo ec eoo peopao a oaaaop.

oa o a aoc eaeo epea ye oec CB-ye eee 17 c CB-oe (paope e pea 52 0C), ae oaaac eeo. Ceeoc poecca cac o 46,5 %ee, o ooo, cao c aae epea, aapyeo eey pea.

Ta opao, oyee ae oaa, o cooae ax oxoo yee cepeoceeoc occaoe AA eeo.

5. oc oax yco occaoe aeoaeaa cceye poopaa oe ceeoc poecca o cceoao e pH cpe, eepayp, oepa cycpaa oacc a aoc cepeoceeoc occaoe AA, ocoy eee eae epe oy oac py o pya oa yco poe coe aoc.

acoc o eepayp 5. p cceoa eepaypo acoc occaoe AA cceye poopaa o ycaoeo, o oac cox x eepayp opayc poy o oeco co.

Haoa oeca coa poya coea c coo apopeyao aoc aaec oox cyax oac eepayp o 32 o 38 0C.

5.2 acoc o pH B peyae cceoa pH-acoc oac ae cooc cpe o 6,0 o 8,0 o ycaoeo, o oy apopeyao aoc oacc aa Pichia sp. 80-11 acaa coa, cepyeoo poya axoc oac 7,5. B cyae aa Metschnikowia sp. 8413 oapyeo, o apaep cee oac pH 6,0 - 6,5 (Pc. 2 ).

5.3 Be oepa cycpaa cceoae aao oepa cycpaa a occaoee AA, oaao, o aoc oacc Metschnikowia sp. 84-13 pyec co oepa eopa. p o aoa aoc ocaec p 5 / AA (pc. 2B).

B cyae pacopa AA ao Pichia sp. 80-11 ocoe ece cycpaa a aoc oacc e aaoc (pc. 2B). Oao, epe 2 - 3 pea apaax c oepae AA oee 5 / aaoc cee copoc pea.

cceoae oeco co poyo, oyex p pacopa AA, ocoo peaoy cec oepax o 2,5 o 15 /, ooo , o Metschnikowia sp. 84-13 o oaae opacae c yeee oepa cycpaa o 2,5 o 5 / capyec a ypoe 72 74 % ee, o pe a Pichia sp. 80-11 oeca coa poya e ac o oepa cycpaa.

5.4. acoc o oepa oacc p cceoa acoc pacopa AA o oepa oacc Metschnikowia sp. 84-13 (o 4 o 16 (ac)/) o oapyeo, o aoee peeo ec oepa ooo 6 (ac)/. cooae oee cox oepa oacc po aeoy ae ee yeo aoc, a peee oee x oepa ce aeca poya.

oacca Pichia sp. 80-11 oe cooaa oee poo aaoe oepa (o 6 o 16 (ac)/), a cee oac x oepa, e aaec cee oeco co poya pacopa.

5.5. cceoae acoc pacopa AA o a aocep eco, o occaoee apocoepax coee ocyecec c oo epeo, coyx aece occaoe NADH NADPH, coepae oopx ee oe cac pcyc copoa, apep, cece oce NADH p yac epopacopo e, coye copo aece aeopa epoo (Nakamura, 2001). o ooo peoo, o occaoee AA cceye aa ye poxo oee eco aocepe aoa, cece oe caoc occaoex op oepeo aapox ycox.

110 110 100 100 90 90 80 80 70 70 60 60 50 50 40 40 30 30 20 20 10 10 0 0 5 5,5 6 6,5 7 7,5 8 8,020 pH Teepaypa, C A 100 100 100 90 90 90 80 80 70 70 60 60 50 50 40 40 30 30 20 20 10 10 0 0 0 10 20 30 40 2 4 6 8 10 12 14 16 oepa cycpaa, / oepa oacc, (ac)/ B oocea aoc aa Pichia sp. 80-oocea aoc aa Metschnikowia sp. 84-Oec xo S-O (a Pichia sp. 80-11) Oec xo R-O (a Metschnikowia sp. 84-13) Pc. 2. acoc aoc oacc ao Pichia sp.80-11 Metschnikowia sp. 84-13 oeco co R-aoepo O o S- apaepo pea * A eepaypa, pH, B oepa cycpaa, oepa oacc * co pea caape, a cee cceyeoo apaepa, aee oopoo appoaoc cepee Oeca coa, %ee Oeca coa, %ee Oocea aoc, % Oocea aoc, % Oeca coa, %ee Oeca coa, %ee Oocea aoc, % Oocea aoc, % Oao, ope oa, oee coa aoc oacc oeaac pcyc oyxa (a. 5). oee oo, epe 3 aaoc aeee copoc pea aocepe aoa, peyae eo oa oepc cycpaa x ycox a ocya oo epe 24 - 48 , oa a pcyc oyxa a 2 - 4 .

aea oyo aocep a aoy cyae aa Pichia sp. 80-11 e pea aeoy ee ceeoc occaoe AA S-O, oa a cyae aa Metschnikowia sp. 84-13 eo eco cee oeco co R-O o 2 paa.

Taa Tpacopa AA O poopaa aocepe aoa oyxa caapx ycox a Cy- co Ooc- oep- Bpe, poy Bxo, Oeca cpa pea ea c, % % coa, aoc, % ee % Boyx 100 100 4 S-O 99 Pichia AA Ao 49 100 24 S-O 100 sp.80-Ao + 43 100 48 S-O 100 Na2S2O Boyx 100 96 2 R-O 76 72,Metschni- AA 11 100 24 R-O 100 43,Ao kowia sp.

Ao + 5,5 100 50 S-O 100 84-Na2S2OO Boyx - 20 10 AA 10 Ao - 0 0 - epeco oe, o eee aapoy peaoy cec R-aa Metschnikowia sp. 84-13 cya ap, ee oee aee poecc pacopa AA po opaoa coocoo S-aoepa O aoa R-aoepa, opayeoo ocyc ao co. B o e pe cyae aa Pichia sp.80-11 cyeceoo cya ap a occaoee AA aeeo e o.

B aapox ycox aac oece xo cooecyx aoepo O a cyae aa Pichia sp.80-11, a cyae aa Metschnikowia sp. 84-13, oa a apox ycox co xo poya oeceaec oo ao Pichia sp.80-11. p pacopa AA a oyxe ao Metschnikowia sp. 84-13, ecop a 96 % oepc cycpaa, xo R-O e peae 76 %, o ooe peoaa aeee pepaee poya poopao.

cceoae pacopa oeoo paeecoo O ao Metschnikowia sp. 84-13 oaao, o eceo, apox ycox ee eco cee oepa oo coee, ocycyee aapox ycox (a. 5). Oao aece poya oapye oo AA (poy oce ocpa), o oop o opaoc cceyex oceo-occaoex pea.

5.6. cceoae e apoo pacopa AA oacco Metschnikowia sp. 84-B peyae cceoa apoo pacopa AA ao Metschnikowia sp. 84-13 eee 40 o oapyeo, o opae a ypoe 76 % xo R-O yeaec o pee o 97 % (pc. 3).

100 80 60 40 20 \ 0 0 0,5 1 2 3 4 5 7 10 24 30 33 Bpe, P2 oeca coa oepc AA xo R-O ea apoo pacopa AA ao Pc. 3.

Metschnikowia sp. 84-B peyae cceoa oecx coc poyo, oyex a paoe pe pacopa, o ycaoeo, o occaoee AA poeae c ecoo aoceeoc ( oe oce paec oo oepc oyaec poy 72 % ee). Taa oeca coa poya ocaec cao eee ecox aco, oao oe pacopa oa aae pac (o 98 %ee). p o oeaec oppe ey eee aoepoo cocaa O opacae xoa poya o e pe.

Boo ocee ooae peaoo cec R-O oe cy cepeoepc oooo S-O peyae aoceeoo oce oo aoepa a oe oceeoo occaoe AA.

O COOC2HCHOH HO H H COOC2H5 COOC2HCH3 CHR-O S-O Bxo (oepc), % Oeca coa, % ee Moo peoo, o o poecc poeae yp e, ocoy eee eoo pee ee eco xo poya o p oo oepc cycpaa.

Ta opao, a ce yee pee pacopa AA ao Metschnikowia sp. 84-13 oo oy cooc R-O apox ycox.

6. Be yco paa a pacopa AA eco, o e oo yco pee oacc poecce pacopa, o yco ee oye, a ae ee opac, e cyeceoe aee occaoe apocoepax coee, ocoy o ooecoo coco poopao oe ace a ypoe epeo, yacyx pacopa, a paep ya occaoex oepeo ee (Dahl, 1998; Faber, 1999).

6.1. acoc occaoe AA o opaca oacc o ycaoeo, o copoc occaoe AA cceye aa oeca coa, opayxc p o poyo ec poecce poca oacc (pc. 4).

120 aoc aa 80-aoc aa 84-60 ppoc oacc aa 80-ppoc 0 oacc 0 16 24 48 72 96 120 aa 84-Bpe, Pc. 4. aa poca apopeyao aoc oacc poopao p pacopa caapx ycox apopeyaa aoc aa Metschnikowia sp. 84-13 ee aoee aee oapeco ae poca (1 2 cyo) aae cac 3 cya epo aee poca (pc. 4). Hapo, aoc aa Pichia sp.80-11 ae aee poca (3 cyo) oo ocae acaoo ypo.

acoc oeco co opayxc poyo o opaca oacc cceyex ao e cpea xapaep (pc. 5).

aoc, % Oocea oacca, o. e.

(S)-O (Pichia sp. 80-11) (R)-O (Metschnikowia sp. 84-13) 24 48 72 96 Bpe, acoc oeco co poyo occaoe AA o oPc.5.

paca oacc poopao p pacopa caapx ycox Haoee c R-O oe oye p cooa oo-, yx- cyoo yyp Metschnikowia sp. 84-13. oye coocoo S-O eoxoo cooa oaccy Pichia sp.80-11 yx pexcyooo opaca.

6.2. Tpacopa AA oacco Pichia sp. 80-11, paeo aapox ycox B peyae cceoa poca cceyex poe poyo x eaoa, aaaxc cpee c caxapoo, aapox ycox o ycaoeo, o Pichia sp.80-11 cocoe pac p opae ocya oyxa, ocyec cpooe poee.

cooae pexcyoo oacc Pichia sp.80-11, paeo ax ycox, pacopa AA aocepe aoa ooo oy a 50 cooc S-O paec c oece xoo (a. 6).

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