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Ha paax pyoc PAMOBA AHHA PMOBHA PAPAOTA OATATECX CCTEM HAHTOCEETBHOO BOCCTAHOBEH APOHCOEPAX COEHEH Ceaoc 03.00.23 oexoo ABTOPEEPAT ccepa a cocae yeo cee aaa execx ay A - 2002 2 Paoa oea co ocyapceo eo execo yepcee.

Haye pyooe: oop xecx ay, poeccop op Bap Bopo;

aa ooecx ay, oe eyxoa Haea aoa.

Oae ooe : oop ooecx ay, poeccop Baxo Beep Acaapo;

aa execx ay peo eop Aecapo.

Beya opaa cy oo coo ayoo e pa PAH.

aa cococ 18 eap 2002 oa 1430 a acea ccepaooo coea 212.289.06 p co ocyapceo eo execo yepcee o apecy: 450062, Pecya aopoca, . a, y. ocoao, 1.

C ccepae oo oaoc oee coo ocyapceoo eoo execoo yepcea.

Aopeepa paoca 18 op 2002 oa.

e cepeap ccepaooo coea Caoo H. A.

3 OA XAPATEPCTA PAOT Ayaoc e. ppoe ooec ae eeca, x ceece aao pooe peca oo epec coa copeex eapcex peapao ooec cx cpec a pace. Ocoeoc oca ppox ooec ax eec ec ceeca cepeox, oycoea ae ox ce xpax epo opeeeo oypa. Coae eoo oye ceecx aaoo ppox ooec ax eec peoaae ocyecee acepecoo cea cooa oec ax oo. co xece oxo pee o aa aco oaac aoe eeecoopa. Haoee epce ec poooec oxo, ocoa a cooa cepeoapaeo opacopa opaecx coee.

Baoe eco cpe poecco cepeoapaeo opacopa aae aoceeoe occaoee apox coee c oo poopao oec ae cp, occo x p, eoxoe oye ooeypx opeyopo cepeopeypx oepo.

ec poopa, poe coy aoceeoc p occaoe oex apocoepax coee. Oao, oco x e cocoo ocyec opacopa c ocaoo coo copoc xoo eeoo poya. aco ee eco opaa cya, oa poopa po coy apopeyay aoc p o aoceeoc, ocoy, a pao, eax eec ecoo epeo, cocox pacoppoa cycpa poy pooooo oypa. Papaoa cooceex eoo occaoe apocoepax coee peye oca cocoo cepeoopo pacopa ye oa cycpaa, oopa opo eax epeo, oa yco pea paa poopao.

B c c oc ox oee ex poopaopacopaopo, oaax poo cycpao ceoc yco, oopx o coco ocyec aoceeoe occaoee poxpax apocoepax peeceo c co xoo ex oec ax coo, ec aae ayao.

ccepaoa paoa oea cooec c aae Mcepca opaoa P o eaecoy ay HP coo ocyapceoo eoo execoo yepcea (1998-2002 .); eepao eeo popao ocyapcea oepa epa ceo opaoa yaeao ay (1997-2001 .).

e pao. Papaoa eoo aoceeoo occaoe apocoepax coee c oo eo poopao, oaax apopeyao aoc, oye ex oec ax coo.

B cooec c ocaeo e peac ceye aa:

cp poopao, oaax coo apopeyao aoc cocooc aoceeoy occaoe poxpax apocoepax peeceo;

cceoae opooecx, ooo-oxecx aoex coc paec ax poopao;

oop yco cea (S)- (R)--3-ocypaa ye occaoe aeoaeaa c oo eo poopao;

oc cocoo cepeoopo cea (S)- (R)--3-ocypaa poopaa;

papaoa aoceex oaaaopo occaoe a ocoe eo poopao;

cceoae yco eo pao oaaaopo;

cceoae ceecx oooce oyex oaaaopo.

Haya oa. Hae epoa oe aoceee a Pichia sp. 80-11 Metschnikowia sp. 84-13, a ocoe oopx coa cepeoceee oaaaop oye oec ax occo cpo ye occaoe poxpax apocoepax peeceo. Papaoa ayo oocoae eo cea oec ax S- R-aoepo -3-ocypaa c cooae coax oaaaopo. Opeee oae apaep pao oaaaopo. Bepe peoe e eo cea S--3-ocypaa c oo ooax eo aa Pichia sp. 80-11, oo oya poy coo oeco co. Papaoa ayo oocoae eo cepeoopo peeepa oaaaopa.

paeca aoc. Papaoa coocepeoceee oaaaop a ocoe ao Pichia sp. 80-11 Metschnikowia sp. 84-13, ocyecx aoceeoe occaoee aeoaeaa S- R-aoep -3-ocypaa coo oeco co. Coa peapae eo cea coocx (S)- (R)- -3-ocypaa (e eee 98 %ee). oye oec c S--3-ocypa cooa aopaop opeyopo aceox OX H PAH cea eco eepoo ooe - epooo aceox - 2S-peaeaa, 2Spe-10E-eaeaa (S)-1-eyox po 2-e- 2,4ee-2E-eox co, oox eo ooec eoaco ocyec ay cecoxocex yyp o aceoxpeee - ooo y (Drosophila mulleri, Drosophila buscki), ecceco yx (Mayetiola destructor) epooo oa (Rhyzopertha dominica).

Meoa aoceeoo occaoe aeoaeaa coyec yeo poecce HT p oooe ceaco o ceaoc 07.01.00 oexoo.

Apoa pao. Peya cceoa oaac a Bopo Meyapoo oepe Ayae ee opaeco cee a opoe oo p (Ca-eepyp, 1999.); XXXVII eyapoo Hayo cyeeco oepe (Hoocpc, ape 1999 .); oepe oox yex (a, ape 1999); Meyapoo ayo-paeco o epe Cepc ooo opoa (a, a, 1999 .); XII eyapoo oepe o poocy pee xecx peao peaeo Pea-99, (a-Moca, cep, 1999 .); Pecyaco ayo oepe .. ao copeee poe oo e, (a, op 1999 .); aoo ayo-paeco oepe "oexoo "epa"", (Ca eepyp, 1999 ); XXXVIII eyapoo ayo cyeeco oepe (Hoocpc, 2000 .); XIII eyapoo oepe o poocy pee xecx peao peaeo Pea-2000; (a-Moca, 2000), XXXXIX eyapoo ayo cyeeco oepe (Hoocpc, 2001 .); XIX eyapoo oepe o poocy pee xecx peao peaeo Pea2001, (a-Moca, 2001) ; Bcepoccco aoo oepe aa oexoo, x xecx exoox (Tep, 2002).

ya. o aepaa ccepa oyoao 3 ca ec 16 oao.

Cpyypa oe pao. ccepa ae eee, oop epayp (1 aa), ocae oeo eoo cceoa (2 aa), cepeay ac ocyee peyao (3 aa), o cco pyeo epayp, coepae 158 cco. Paoa oea a 115 cpaax aocoo eca coep 20 pcyo 19 a.

oeceoo opeee oepa opaecx cycpao poyo pea, x ea oe oecx coc pe eo aooco xpoaopa, xpoaoac-cepoep, MPcepoco opep.

PETAT OCEHE 1. Cp poopao c apopeyao aoc oc poopao, oaax apopeyao aoc, ocyec cpe ao, eex oex opao, x c pax yaco ecoapoo o . , eeepepaaax pooc Pecy aopoca eepoco Cp. poe oo, aece oeax oaaaopo cac oapa oacca eapcx poe, ycaea a yco pooceo oee "ao", a ae ceepaee e x poe poe Candida utilis -654, coyex p pooce B.

B aece pacoppyeoo cycpaa cooa aeoaea (AA), oec ae poy occaoe oopoo, S--3ocypa (S-O) R--3-ocypa (R-O), pec cee epooo aceox, coyex a cecoxocex yyp, cee ox aepao xpyp, opaaaex oepo, paec ex opeyopo (apoa B, RR-peoopa, paa A, cyaoa, oeooa peceoa), a ae aepao-aocao eo poeoc.

B peyae ecpoa 102 poex ao ceyx ycox: 0,05 M oca yep, pH = 7,0; cycpa 5 /; oacca 8,5-(ac)/; apoe yco, eepaypa pea 30-32 0C, pe pea 4 , e 17 ao, cocox oeppoa AA oee e a 40% (a. 1).

Taa Peya occaoe AA poopaa a oepc Bxo a oepc Bxo AA, % O, % AA, % O, % 80-1 76,0 75,1 81-13 90,2 88,80-2 80,4 69,5 82-2 45,7 44,80-11 100 99 82-16 96,0 20,80-12 74,3 70,8 82-18 85,4 15,81-1 80,0 77,5 83-2 77 21,81-2 50,7 49,7 83-3 100 19,81-3 40,0 35,9 84-13 100 90,81-11 79,2 78,8 85-13 69,1 67,81-12 99,3 97,Candida utilis 89 eapce po- - oape 30,4 29,p o oco ao aaa poecce pacopa O c co oece xoo. cee coca a, ayypye poy eox oecax aapye eo po:

a 82-16, O Bce a OH OH 82-18, 83-2, 83-+ C2H5OH COOC2HCOOC2HCOOH CHCH3 CHcceoae oecx coc poya pacopa oaao, o ce aee a ocyec occaoee AA aoceeo (a. 2).

Taa Oece coca O, cepoaoo cceye aa Oeca Oeca []D []D a coa, (oy- coa, a (oyp % ee % ee pa) a) 80-1 -34,5 (R) 75,0 81-13 +30,2 (S) 70,80-2 +37,4 (S) 87,0 82-2 +6,5 (S) 15,80-12 +18,2 (S) 42,4 82-16 +7,4 (S) 17,80-11 +40,9 (S) 88,0 82-18 +9,4 (S) 21,81-1 -25,8 (R) 56,0 83-2 +32,3 (S) 75,81-2 +25,3 (S) 62,6 83-3 +30,9 (S) 72,81-3 +17,4 (S) 58,7 84-13 -33,2 (R) 72,81-11 +21,0 (S) 48,9 85-13 -9,1 (R) 19,81-12 +38,6 (S) 85,7 Candida utilis -654 + 30,2(S) 65,eapce +5 (S) po o ycaoeo, o oco ao cepy S- O oo 4 aa opay R- O.

a O a OH R-a S-a H HO H COOC2HCOOC2HCOOC2HCHCHCHS-(+)-O R-(-)-O cceoae e oeceo oe apopeyao aoc poopao oaao, o aoee ao occaaa AA a 80-11, 81-12 84-13 (pc.1).

a S-a a R-a 2,1,0,a ea aoc occaoe AA aoee a Pc. 1.

aa Ceye oe, o x aoc peaa peyay aoc oacc eapcx poe 30-36 pa.

Ta opao, aoee epce c a aa S-a (80-11, 81-12) o a R-a (84-13).

2. ea cceoae aoex coc epcex ao Ha ocoa peyao cceoa ocox opooecx ooo-oxecx pao aee poopa epoa a Pichia sp. 80-11, Candida sp. 81-12 u Metschnikowia sp. 84-13.

cae x ao a aoeoc, oeoe copya cya oo pya e eoea Pecy aopoca oaao, o o e oaa aoe eoea ox coca.

ea aoc, Mo/( 83-83-80-80818184 3. cceoae occaoe pa apocoepax coee aoee epcex ao poopao, ex o xapaepca occaoe AA, a poeea oea cocooc occaoe pa apocoepax cycpao pax o po ocoapoox co (AA), aeoo (eao-2, (3S)-3,7-e-6oeo, eopyeo), xopcoepax coee oeaooo pa (8-poc-1,4-oca-6,8,9-pxop-cpo/4,4/o-6-e-8-o, oeao), ecx eocoepax (3,3-e-2-oco-ypoao). cceoae ooc caapx ycox: 0,05 M oca yep, pH = 7,0;

cycpa 5 /; oacca 8,5 (ac)/; apoe yco, eepaypa pea 32 0C, pe pea 4 ..

pooe oeaooo pa c a coa cee pocaoo, pocaao poooyxoex peapao. caoeo, o cceyee a oaa coo apopeyao aoc ooceo 3,3-e-2-oco-ypoaoa, oeaoa eopyeoa. p occaoe 1,4-oca-6,7,9-pxopcpo/4.4/o-6e-8-oa, ecop a 47% oepc, 1,4-oca-6,7,9-pxopcpo/4.4/o-6e-8-oa peaoo cec oapyeo e o (a. 3). Booo, ao cyae ee eco cpa aea oepc opayeoc poya pyo, eoccaoe y pacopa cycpaa. poe oo, ae oeye oo coee, aoo xopa e cae eo aoec c a pya eo.

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