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O O OH OH apaxooa coa: 20:4 (n-6) oeoa coa: 18:1 (n-9) O O OH OH -oeoa coa: 18:3 (n-6) oaeaeoa coa: 20:5 (n-3) O O OH OH oaeoa coa: 20:2 (n-9) oo--oeoa coa: 20:3 (n-6) O OH oea coa: 18:2 (n-6) oyee peya oo cea o o o, o yeee o eacex ocoo pocxo a ce aoe A, pe acaoe oeco ocee pxoc a epa ee pHa.=7,08,0 (pc. 5). acex co aox oecax aaaac ecaeaoa coa (o 33% o cy p pHa.=3,0), coepae oaeaoo co ao eoc o 8,4% (pHa.=3,0) o 15,8% o cy (pHa.=11,0).

3.2. Be eepayp paa a xo px co oacc Mortierella alpina 18-B peyae cceoa eepayp a poc ce H po Mortierella alpina 18-1 o ycaoeo, o c poco eepayp epae 730 0C yeaec xo oacc (pc. 6). a acoc ee cpea xapaep c acyo p 2030 C. B o e epae aaec aca xo H, xo eo ceeoc x opaoa cpe cy opacae c oee eepayp.

cy Ocoe eacee , % o 60 40 20 0 5 Teepaypa, 0C cya oacca Pc. 6.

Bxo oacc cy px co acoc o eepayp paa po-co coca aeo ac o eepayp paa (pc. 7). Ta, c oee eepayp ypoa yeaec ceeoc opaoa acex , oa a p ee oe opacae ceeoc opaoa eacex . ooe ee po-cooo cocaa c o cocoo pey eyec oo co oo ocyecc, acoc o poopaa, pa ecaypaa. Ceye oe, o p oeo eepaype paa (7 C) cpe px co aoo poopaa oapye ocpoe (C12, C14 C16), 2-pocecaeaoa coa, Z9 - ecaeaooeoa coa eoope pye opooeoee eacee coee, oy cpyypy oopx oa e yaoc ycao.

5 Teepaypa, 0C.

eacee acee ocae coee Pc. 7.

Coooee py coee acoc o eepayp paa oacca, (ACB)/ Cya , % o ACB py coee, % o cy pe eyec oo co Mortierella alpina 18-1 p x eepaypax ocoo peypyec ye yee , a ae yeee cee eaceoc oeoex (pc. 8). p oe eepayp paa c 25 o 30 0C cpe , cepyex a po, yeaec o eacex ( ocoo a ce A), p o yeaec o ee peecea, oo--oeoo co, c 4,9 (25 0C) o 6,1 % o cy (30 0C). Oopeeo c opace coepae acex oeoex (% o cy ) oaoo (o 1,2 o 1,5), ooaoo (o 3,0 o 4,3) epaoaoo (o 5,3 o 9,3).

5 Teepaypa, 0C 18:1(n-9) 18:2(n-6) 18:3(n-6) 20:5(n-3) 20:4(n-6) 20:3(n-6) 20:2(n-9) 24:1(n-9) Pc. 8.

eee cocaa eacex px co acoc o eepayp paa Hao xo A oeceaec p paa pa Mortierella alpina 18-1 epae ee eepayp paa 2025 0C.

3.3. Be pee ypoa a xo oacc px co Mortierella alpina 18- oyex ax o ae aoe oacc o o pee (pc. 9) o, o pa aoe oacc ee epoeco yyp xapaep. Haoee oeco cceyea yypa Mortierella alpina 18-1 aaae ae eoo poca. B aee, o pe a caoapoo poca, cyapoe coepae paec e eec aae ao cac a oce cy o ~42% o ACB.

cy Ocoe eacee , % o 50 0 40 64 88 112 136 160 184 Bpe paa, .

cya oacca Pc. 9.

aa aoe oacc Mortierella alpina 18-1 o pee Ceye oe, o opacae oeo ypo cyapx pocxo a ce aoe eacex yee o acex (pc. 10).

40 64 88 112 136 160 184 Bpe paa, ac eacee acee ocae coee Pc. 10.

aa ee py coee Mortierella alpina 18-1 o pee Bao oe, o yeee ypo cyapx poecce poca pa ocoo pocxo a ce yee coepa x A (pc.

11).

oacca, (ACB)/ Cya , % o ACB py coee, % o cy 40 64 88 112 136 160 184 Bpe paa, .

18:1(n-9) 18:2(n-6) 18:3(n-6) 20:5(n-3) 20:4(n-6) 20:3(n-6) 20:2(n-9) 20:1(n-9) Pc. 11.

aa aoe ocox eacex Mortierella alpina 18-1 o pee p o coepae A peae coepae ao oeo eaceo a cee cy poca peoaae cpe eacex Mortierella alpina 18-1, pe acy ee aoe pxoc a acy aoe cy (pc. 9, 11).

3.4. Be ooe oa oey cpe a xo oacc Mortierella alpina 18-caoeo, o c yeee ooe oa oepxoc oey aeo cpe pocxo eca poecca aoe oacc coepa e (pc. 12), o oe oceo ooce yeee copoc accooex poecco.

6,00 60,oacca cya 5,00 50,4,00 40,3,00 30,2,00 20,1,00 10,0,00 0,1,41 1,77 9,Ooee oa/oe, c2/cPc. 12.

Be ooe oa oepxoc oey aeo cpe a poc aoee Mortierella alpina 18-Ocoe eacee , % o ACB Cya , % ACB oacca, (ACB)/ oyee peya oaa, o eee po-cooo cocaa acoc o ooe oa oepxoc oey aeo cpe eaeo, coepae ocox eacex ae paec e eec (pc. 13).

1,41 1,77 9,Ooee oa/oe, c2/c18:1(n-9) 18:2(n-6) 18:3(n-6) 20:1(n-9) 20:2(n-9) 20:3(n-6) 20:4(n-6) 20:5(n-3) Pc. 13.

eee po-cooo cocaa Mortierella alpina 18-1 acoc o ooe oa oepxoc oey aeo cpe 3.5. Be oeca oya eo a a poc poyea ce px co p cceoa oeca a oya a poc poyea ce o ycaoeo, o aoee oeco oacc opayec cyae cooa aece oya cyce cop paeo e, oyeoo co cepo oopooo oo co coeo popax cpe aea (pc. 14) oece 2,5 % o oea aeo cpe.

oacca 7 cya 6 5 4 3 2 1 0 e+ e+ e+ cop cop cop cop cop cop (1%) (2,5%) (10%) (1%) (2,5%) (10%) B oeco oya Pc. 14.

Be a oeca oya a poc opaoae Mortierella alpina 18-cy Ocoe eacee , % o oaca, (ACB)/ Cya , % ACB Ooceo oee aoee a oacc, a o cyae oe occ copaee pooeoc a-a poca pa.

3.6. oc cyopo poca poyea cea apaxooo co eco, o ae coee a e, oycyca coa (), pocep, oaa cypyee ece a poc pae pace poopao. B peyae poeex cceoa o oapyeo, o cyae cooa ea pocepa yeaec xo cyapx , o cpae c opo oo, cocae cooeceo 103,7 134,4 % (pc. 15). oyee peya oaa, o cyae cooa xo oacc oaec (127,9 % o opo), a xo cyapx caec cocae 76,7 % o opo.

B cyae ece aey cpey ea pocepa ooceoe eee ppoca oacc cooeceo cocae 108,6 91,4% o opo.

4 oacca cya 3,2,2 1,0,0 opo e pocep Pc. 15.

Bxo oacc cy p ypoa Mortierella alpina 18-1 a cpeax c pa cyopa p cceoa cocoa eca cea cy H, o ce A, y pa Mortierella alpina 18-1 ycaoeo, o cyae pee cex yaax cyopo yeaec acooe coepae A pacee a ey oea cpe (pc. 15, 16). p o acooe yeee xoa A ocae acaoo ae cyae pee ea cocae 158 % o opo. Aao oaae pocepa coca cooeceo 121,4 136,4 % o opo.

oacca, (ACB)/ Cya , % o ACB opo e pocep 18:1(n-9) 18:2(n-6) 18:3(n-6) 20:2(n-9) 20:3(n-6) 20:4(n-6) 20:5(n-3) Pc. 16.

Coepae ocox eacex Mortierella alpina 18-Ta opao, peee yaax cyopo poca oeceae oee ypo xoa A pacee a o p aeo cpe a 41% (e), 35% (pocep) 8% (). p o ceeoc opaoa A cpe , cepyex a poopao, yeaec eaeo (pc. 16).

3.7. eca cea apaxooo co ye paa oepxoc cocoo a epo cpee a o peyx ax, oa yco paa pa Mortierella alpina 18-1 oepxoc cocoo a ooapoeo cpee c: eepaypa paa ~25 0C, pe paa ~168 , aaa pH cpe ~67, cooae aece oya eeoo e oece 2,5% o oea aeo cpe. Oao p paa x ycox xo A pacee a ey oea cpe ec eocaoo co (~11,5 (A)/). po oo oe cy e p aopo: eocaoo ooe ooee oepxoc cpe ee oey ( o copoy), eocaooe oeco cycpaa ( o cycpay) , pe cyae, cooae aece oya cyce cop. ycpae x eax aopo, ypoae ocyec a cpee, coepae 600 apoe a 1 cpe 6% o (coa cycpaa 2 ). p o ae a eee 19 cyo aaa 22,4 / . cceoae po-cooo cocaa oaao, o cpe , cepyex ao, x ycox peoaae A (~48%) (pc. 17). Ceye oe, o acex aoe oece aaaac ceapoa coa (~ 19 % o cy ). Cpe ae oapye eoope poc- pooe C14-C16 .

Heacee , % ACB 30 25 20 15 10 5 0 3 5 7 9 11 13 15 17 19 Bpe paa, .

cya 18:1(n-9) 18:2(n-6) 18:3(n-6) 20:4(n-6) 20:3(n-6) Pc. 17.

aa aoe cy ocox eacex po Mortierella alpina 18-1 p oepxoco ypoa Bxo A p cooa oepxocoo cocoa ypoa coca 10,8 / aeo cpe.

4. oyee poox apaxooo co H oy pec a cooe , a ae e ox po a oo-, - paep. Cooe oee peoe ee cepeo-cocycx aoea, a coe oe p coyc peopae paa ee, ooeex cyopo ax pea, poca pa pace. Ta, apep, ycaoeo, o paep - aoee peea opa apaxooo oooecaoo co cooa a pyx ee, ocoy oe co xo coca aepcoo ooa eo e paepo.

4.1. oyee eooo pa apaxooo co oyee eooo pa A poo coaco eco eoe epoa c cooae aexopa eaoa, peyae eo a oyea coa cec eox po . oyee eoe p oepy paopoa c oo oe oye oepaa A.

oyee peya oaa, o paopoae eox po Mortierella alpina 18-1 ooe oya oepa A c ee coepae o ~73% o cy (a. 2).

% o cy Heacee , Cya , / cpe Taa Coca oepao eox po , oyex p coooe oea/ 4:1 cpaa pa Mortierella alpina 18-1, pacope - eao , % o cy o opao oce opao 14:0 4,25 0,15:0 0,31 16:0 15,56 0,17:0 0,21 18:0 5,48 0,20:0 0,64 18:1(n-9) 4,32 0,18:2(n-6) 4,42 2,18:3(n-6) 4,12 10,20:5(n-3) 2,15 6,20:4(n-6) 50,81 72,20:3(n-6) 4,25 6,20:2(n-9) 1,37 0,20:1(n-9) 1,99 acex 26,45 1, ooeacex 6,31 0, A, , 57,21 85,oye oepa A oo cooa execx ex, apep, coa a eo ocoe cyopo poca pace. Oao oye cooco A, coyeo ex ex, eoxoa ooea oca, apep, c oo cooeo oco xpoaopa.

4.2. oyee cox po epa apaxooo co Papaoy eoa oye pa A epa poo a aa: a epo ae poee cp poopao, oaax epeao aoc o ooe eooy py A, a opo ae ocyece oop pacope poee cea cooo pa A epa.

4.2.1. Cp poopao, oaax epeao aoc eooy py apaxooo co Oea cceoa c poopa oe aep ox exoo poooecx pooc HT, poe coy oecy aoc p poe oooo aca, a ae a, oaae coo aoc ( opaeco pacopee ecae) p epa eaoa-2 oeoo, ecaoo eaoo coa (a. 3).

B peyae cpa poopao, cepyx epe oope po cycpay ceoc eooy py A, ae a (Bacillus sp. 77-1, Pseudomonas sp. 77-33 Pseudomonas sp. 77-47), aapye aoo yaaoo pa -yaoo. o ycaoeo, o poecc peepa aoee ao ocyece a Pseudomonas sp. 77-33 p cooa aece pacope peyaoa.

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