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Ha paax pyoc ABETAEB OP MAPATOB OCHTE OHEHACEHHX PHX COT X POBOHX Ceaoc 03.00.23 oexoo ABTOPEEPAT ccepa a cocae yeo cee aaa execx ay A - 2002 2 Paoa oea co ocyapceo eo execo yepcee.

Haye pyooe: oop xecx ay, poeccop op Bap Bopo;

aa ooecx ay, oe eyxoa Haea aoa.

Oae ooe : oop ooecx ay, poeccop Meee Aecap ao;

aa ooecx ay eo a o.

Beya opaa cy opaeco x coo a yoo epa PAH.

aa cococ 18 eap 2002 oa 1600 a acea ccepaooo coea 212.289.06 p co ocyapceo eo execo yepcee o apecy: 450062, Pecya aopoca, . a, y. ocoao, 1.

C ccepae oo oaoc oee coo ocyapceoo eoo execoo yepcea.

Aopeepa paoca op 2002 oa.

e cepeap ccepaooo coea Caoo H. A.

3 OA XAPATEPCTA PAOT Ayaoc cceoa: oeacee pe co (H) peca coo ya acc opaecx eec, pax ay po ooecx cceax. cceoa ocex pex ece cp po cep x y x opaax. H oepac opacopa oceaa ooceaa, o po opaoa oocex ooeypx peyopo poecco, poeax eax, ax opae eo. Oo cax ax H ec apaxooa coa (A), oopa cyae po eocpeceoo peecea cep pocaao, eopeo poocao. Oco oac pee A c: apaoo (peece pax eapcex poaecx peapao peex p aoeax cepeo-cocyco cce, ee p.); oceeca poeoc (cpeca o yxoy a oe); ea poeoc (ooaee pax poyo a, o ce cyccex ecx oox cece p.); cecoe xoco (cooe cyop poca ax pea pace) p. B acoee pe oco coo oye A c e cpa ee c pyx opao ox, o eae x pyoacaoe pooco ee (coepae A ocae e oee 0,2% epecee a cyxy accy). B eee ocex aa e aee ycex ocy oac oexooecoo oye A c oo x po opcx oopoce, oope pe cyae oo ocyec ee poeoe pooco. Oao cyecye a ceo e oexoo oye A ae o coepeca, ocoy ee xo aopaopx ycox yx cyax cocae 13 / (o), a cpee y pax cceoaee ooo 610 / (Pocc, CA, oa p.). B c c aya ec oc coae oeecex poyeo A ex oexoo ee oye a x ocoe. aa paoa a oea o aa aex HP coo ocyapceoo eoo execoo yepcea (HT) cooec c eepao eeo popao ocyapcea oepa epa ceo opaoa yaeao ay a 1997-2001 . 20022006 .

e pao: oyee ox cooex poyeo A, oc yco x eoo ypoa c e oe xoa eeoo poya. Coepecoae oexoo oye A. Coae eoo ocea paec ax eec a ee ocoe.

B cooec c ocaeo e peac ceye aa:

cp poopao oeax poyeo A;

cceoae opooecx, ooo-oxecx aoex coc aex epcex poyeo;

cceoae yco ypoa epcex ao a xo oacc, o A;

oc yco ocea cox po A epa c oo oecx poopao;

oocoae pao cxe oye A c cooae aex epcex ao.

Haya oa: poee cp poopao, peyae oopoo ee epoa o cooe a - poye A Mortierella alpina 18-1. Bepe cceoao e yco ypoa pa Mortierella alpina 18-1 a copoc ceeoc ocea A. Hae apaep ocyece eoo ocea A c xoo 10,8 / cpe p eepaype 2025 0C, pHa.=6,57,0 pee ypoa 19 cyo a ae aa Mortierella alpina 18-1. Ha ocoe cceoa poeyox eaoo, acoc x xoa o yco ypoa peoea oeeca cxea ocea H y cceyeo yyp pa Mortierella alpina 18-1. caoe yco cea cooo pa A epa c oo oecx poopao.

paeca aoc: Papaoa cooe eo oye A c oo eeoo poyea Mortierella alpina 18-1 ayo oocoaa oexoo ee oye. oaaa eoc papaoao oexoo, oeceae oooc oye A c xoo o 10,8 / cpe. Papaoa eo ocea cooo pa A epa, coyeoo apaoo, ocee. Peya ayx cceoa e ocoy coa ox aopaopx pao coyc yeo poecce ooo ceaco o ceaoc oexoo.

Apoa pao: Peya cceoa ooe a: ayopaeco oepe oexoo epa (Caeepyp, op, 1999); pecyaco ayo oepe .. ao copeee poe oo e (a, op, 1999);

Meyapoo ayo-paeco oepe, oceo 425-e .

, Cepc ooo opoa (a, a, 1999); II Meyapoo oepe oox yex Ayae ee opaeco cee a opoe oo p (Ca-eepyp, , 1999); XIII Meyapoo ayo-execo oepe Xece pea, peae poecc aooao x (Tya, a, 2000); 51 ayo-execo oepe cyeo, acpao oox yex (a, ape, 2000); XXXIX Meyapoo ayo cyeeco oepe Cye ayo-exec popecc (Hoocpc, a, 2001); 52 ayo-execo oepe cyeo, acpao oox yex (a, ape, 2001); XIV Meyapoo ayo-execo oepe Xece pea, peae poecc aooao x (a, , 2001); ayo-paeco oepe X exoo pee peyopo poca pace (a, eap, 2001); oe-ceape Xeca oo (a, op, 2001);

oepe X exoo pee peyopo poca pace (a, eap, 2001); Bcepoccco oepe aa oexoo, x xecx exoox (Tep, a, 2002).

ya: o aepaa ccepa oyoao 13 ayx pao, o ce 1 ca ec 12 oao.

Cpyypa o pao: ccepa ae eee, oop epayp (aa 1), ocae oeo eoo cceoa (aa 2), cepeay ac ocyee peyao (aa 3), o cco pyeo epayp, coepa 128 aeoa. Paoa oea a 102 cpaax aocoo eca coep 29 pcyo 10 a.

PETAT OCEHE 1. Cp poyeo apaxooo co oc ox ao poyeo A ocyecc cpe oeo, y oopx acoey pee oapye cae ae ce A. Op cp po pooc pax o aopocaa ocyecc a aa (pc. 1).

Oop oex opao (87 opao) cpa cop cepo cpoao oe Bpaae a cpe- Bpaae p e c oaee e- eepaype 5 0C a CP-1 CP-2 CP-3 CP-Cp p eepaype 25 0C Bpaae a cpee c pao oepae acpa a, pacye a, e pacye p oepa ac- p oepa acpa 0,84 / pa 0,84 / Bop aoee oeoeoo aa c ao coepae A ax Pc. 1.

Oa cxea cpa po oeax poyeo A:

aee cpe CP-1, CP-2, CP-3 CP-4 cooeceo caxapoo-apoea, oo-apoea, cyco+caxapoa, cyco+oa.

yyp 1 a: eee cx o 2 a: oop aoee acpycex a Ha epo ae aoex yyp o eeo oee ao x oex po. B aece ceeoo aopa, oae coycye aepao op, aae pao cooac e. oe oaee ea cpey o aeeo a paae p oeo eepaype, ocoy o cyae pocxo oaee coycye po aepao op, e coepae ax-o aex oec H cox oaaecx epaax , ceoaeo, e cocoo pac oox ycox (Ape oxa p. 1999).

Ha opo ae cpa poyeo A cooa eo, peoe Epo B. . c coaopa, oope p paoe c oea poa Mortierella oapy oppe ey ypoe cea A yceoc poca po oepa acpa (ooo 0,84 /).

B peyae cceoa oopa 22 aa oex po, opayx xapaep oeo ecepoa e, oaax oeo yceoc acpy. cceoae - opaye aoc x ao oaao, o aoee co cyap coepae px co () xapaepyc a 50-212, 51-202 18-1 (pc. 2).

cya o ACB A o cy a Pc. 2.

Coepae A y acpycex ao Aa cocaa oyex o eoa MP xpoaoacccepoep (oyee cep cpaac co cepa eyapox a ax o a www.lipid.co.uk http://lipid.bio.m.u-tokyo.ac.jp) oaa, o coepae A o cy cocao y aa 18-1 - 55%, ocax cyax ee coepae e peao 15% o cy (a 24).

Tae ceye oe, o a 67-1 (peapeo epoa a oe, oocc poy Mortierella) aaa o 27% oo--oeoo co o cy , oao, c yeo eo o oeo aoc, paecoe peee aoo aa poeoo cooa eeecoopao. Ta opao, peyae opoo cpa poopao ee o a, coco aaa o 41% o ACB, 55% oopx pxoc a A.

61-60-31-80-18-51-51-32-80-5c 85-25-60-51-2c 21-75-51-25-32 25-34 25-31 50-2151-202 2. ea aa 18-1. poepa eo aoeoc Ocoe opooece xapaepc pa, eoxoe eo ea, oee a. 1.

Taa Ocoe opooece xapaepc aa 18-pa Ocae pocxoee Pocepa oo e P, c pao.P Be oo Poea ex oace.

Boy e popa ex oacx.

Cycpa e e aoopa, o, peoaae. B oax, po ooo 1 c. B e oo aaocoo.

a copaeoce 50100 aep:

- ocoa copaeoca 45 - ep copaeoca 1,22 Paep:

- copa 810 - cop 234 - cocop 3540 Cocop Oe, ococee, opye Ocoe pa: aax poopoo pa, oeeo coco pacpocpa e a ce cocyo, oopx p paaec, a pee cycpaa p poce o ao cpee pa axaa. Ta opao, o cye opooecx pao a poopa oece poy Mortierella y alpina.

B peyae cceoa aoeoc co H e pya oo eoea ycaoeo, o cceyea yypa Mortierella alpina a 18-1 apyea eoax, e poe eooc, aoc oceoc.

3. yee yco ypoa Mortierella alpina 18-1 a oce oeacex px co C e oca aoee ex yco ypoa oye acaoo xoa eeoo poya A yaoc e aaoo ae pH cpe, eepayp pee paa, ooe oa oey cpe, oeca oya eo a a poc ce o pa Mortierella alpina 18-1. Cacecy opaoy peyao ocyec a epcoao oepe c cooae popa MathCAD 2000.

3.1. Be aao cooc cpe a xo px co oacc Mortierella alpina 18-oyee peya oaa (pc. 3), o eee aaoo ae pH aeo e a a xo oacc, a a coepae e .

60 0 2 3 4 5 6 7 8 9 10 11 Haaoe aee pH cpe cya oacca Pc. 3.

Bxo oacc cy px co acoc o aaoo ae pH cpe Haoee oeco opaoaoc p pHa.=6,57,0. B o e pe aee ee aac po-coo cocae o cceyeoo poopaa (pc. 4). Ta, aoee oeco eacex opaoaoc p pHa.=6,58,5.

2 3 4 5 6 7 8 9 10 11 Haaoe aee pH cpe acee eacee ocae coee Pc. 4.

Coooee py coee acoc o aaoo ae pH cpe Ceye oe, o epae ee pHa.=3,04,0 cpe , cepyex a ao, oapye 2-pocecaeaoa Z9 - ecaeaooeoa co, pHa.=8,311,0 - 2-pocecaeaoa coa, a p pHa.=3,0 - E9-oaeaooeoa coa. B epae pHa.=3,06,0 cpe a oapyea ae Z9 - ecaeaooeoa coa. Hae x coee cocae cceyeoo pa, epoo, opaae aay pea epax o cpea yco cyecoa.

oacca, (ACB)/ Cya ,% o ACB cy py coee, % o 2 3 4 5 6 7 8 9 10 11 Haaoe aee pH cpe Pc. 5.

eee cocaa eacex px co acoc o aaoo ae pH cpe:

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