356 инфекционные БолеЗни Table 2 Table Different parameters important for the structural Docking energy of different screened ligands with the assessment, including chi and Phi-Psi angle distributions predicted protein conformation is tabulated, showing is scored, showing that the predicted conformation is the that P1-(5’-Adenosyl) P4-(5’-(2’-Deoxy-Thymidyl) Tetra native state phosphate was best screened ligand as it showed the lowest Docking Energy of -9.238 Kcal/mol parameter Score average Score ligands docking energy Dihedral Angles p1-(5'-adenosyl)-p4-(5'-(2'phi-psi distribution.– 9.238 kcal/mol deoxythymidyl))-tetraphosphate chi1-chi2 distribution -0.adenosine-3'-5'-diphosphate – 7.955kcal/mol chi1 only 0.05 -0.9-hyroxyethoxymethylguanine – 7.26kcal/mol chi3 and chi4 -0.9-(4-hydroxybutyl)-n2-phenylguanine – 8.54kcal/mol omega -0.ther analysis . docking time utilized was from 145 to Main Chain Covalent 290 seconds, showing that these ligands can effectively Forces act on target protein in a very limited time period. dockMain-chain bond lengths -0.ing energy parameter for different ligands is tabulated -0.Main-chain bond angles -0.below in table 3 and the structural conformation of these ligands is shown in figure 4. Structural detail of best overall average -0.docked protein with ligand is shown in figure 5.
Discussion. ncbi server was first used to get fasta chimera . Model is shown in ribbon structure in figure 3, format of -tif, then by blast against pdb we got temwhere secondary structures are denoted by different colplate for our protein, and verified the result with the help ors like; helix-red, strand-green, light blue-turns .
of pdbsum server . then after template identificaDocking tion, alignment was done between target and template to trace the cure for hSV-1, different ligands were by using align2d.py file of modeller9v5. after that by usscreened for herpes virus considering the pdb as a ing model-single.py file of modeller9v5 we generated lignad depository source . these ligands were then 100 models to verify the quality of generated models screened out on different parameters. first, ligands bindprocheck was done. by procheck 10 plots were genering to herpes capsid protein were screened and all the ated for every model, by comparing these plots, it was ligands sharing similar properties were sorted out. comconcluded that model 13 is the best structural conformon ions, which are already reported as ligands in pdb mation. then energy minimization of model 13 was atwere then rejected. then, the flexible docking studies tempted by swiss pdb viewer, and finally best model of the protein with the selected ligands were carried out structure was accessed by using various other servers using the arguslab docking software . all residues like whatcheck, etc. to check the compactness of strucof ligands were considered for doing docking studies. it ture. flexible docking was then attempted by arguslab was done by searching 106 to 150 different poses of the software because of its various interactive features like ligands, showing that these sites could be used for furretrieving all possible poses where ligand can bind to a Figure 5: (A) illustration of the docking result of best fit ligand p1-(5’-adenosyl) p4-(5’-(2’-deoxy- thymidyl)) tetraphosphate (shown in blue and yellow) with protein -tif (shown red). it is clearly visible that ligand binds at outermost portion of protein making it a better future drug. (B) closer view of docking confirming that the ligand is better entangled in outer region of protein efficiently Саратовский научно-медицинский журнал. 2010. Том 6. № 2.
INFeCtIOUS DISeASeS 8. http://www.ncbi.nlm.nih.gov/protein/protein. thus, one can carefully select different residues 9. http://www.ncbi.nlm.nih.gov/sites/entrezcmd=Retrieve&d from the protein to look for specific binding site. after getb=protein&dopt=genpept&Rid=y8uf7yy4011&log%24=protto ting docking result in form of dock energy, it can be easp&blast_rank=1&list_uids=ily concluded that adenosyl terta-phosphate is the best 10. http://www.rcsb.org/pdb/explore/pubmed.dostructureid= screened ligand for the desired protein conformation.
2q9t Conclusion. -tif, a herpes simplex virus type 11. http://hoppscore.lbl.gov/ (hSV-1) tegument protein, in association with cellular 12. http://swift.cmbi.kun.nl/gv/whatcheck/ 13. arguslab 4.0.1, Mark a. thompson, planaria Software proteins, trans-activates viral immediate early genes.
llc, Seattle, wa, http://www.arguslab.com in order to examine the role of -tif during acute and 14. ace c.i., Mckee t.a., Ryan J.M. et al. a herpes simplex latent infection, the structure of -tif protein is too imvirus type 1 mutant containing a nontransinducing -tif protein portant . this structure can be used further for drug, establishes latent infection in vivo in the absence of viral replicavaccine or antibody designing. So predicted structure of tion and reactivates efficiently from explanted trigeminal ganglia.
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web&page_type=blasthome удК614.23:616.31-089:616.98:578.828niV]-035.2(669)(045) Оригинальная статья original article OCCUPAtIONAL RISk OF HIV INFeCtION AMONG DeNtAL SURGeONS IN NIGeRIAN C.Ch. Azodo – Nigeria, Edo State, Benin City, University of Benin Teaching Hospital, Department of Periodontics, Senior Registrar/Associate Lecturer, BDS, MSC.
риСк Заражения вич – инфекцией У ХирУргов-Стоматологов нигерии в ПрофеССиональной Среде К.Ч. Азодо – Нигерия, Университет базовой клиники г. Бенин, отделение пародонтологии, старший ординатор, бакалавр стоматологических наук, магистр.
data received – 18.02.2010 г. дата принятия в печать – 15.06.2010 г.
C.Ch. Azodo. Occupational risk of HIV infection among dental surgeons in Nigerian. Saratov Journal of Medical Scientific Research, 2010, vol. 6, iss. 2, p. 357-360.
Background: prevention of accidents and management of exposures in the work environment is an important occupation health issue. this study objective was to investigate the occupational risk of hiV among nigerian dental surgeons. Methods: this descriptive cross sectional survey of 300 dental surgeons practicing in private and government owned dental centers in nigeria was conducted from June 2006 to January 2007. Results: percutaneous injury was recorded among 69.3% of respondents and only 1.2% had post exposure prophylaxis. those with abraded skin that will treat patient without additional barrier were 8.6%. percutaneous injury was positively related to gender, position, additional qualifications (p<0.05). Conclusion: percutaneous injury is significantly high and low preventive measure at such exposure. policies, practices, and trainings geared towards protecting and reducing the prevalence of percutaneous injury among dental surgeons, and improving post exposure prophylaxis uptake in the event of exposure is a necessity Keywords:, dentist, infection, hiV, occupational risk.
К.Ч. Азодо. Риск заражения ВИЧ-инфекцией у хирургов-стоматологов Нигерии в профессиональной среде. СаРиск заражения ВИЧ-инфекцией у хирургов-стоматологов Нигерии в профессиональной среде. Саратовский научно-медицинский журнал, 2010, том 6, № 2, с. 357-360.
Сокращение количества инцидентов в профессиональной среде является важной проблемой современного здравоохранения. целью исследования представляется изучение фактора риска заражения ВИч-инфекцией среди хирургов–стоматологов Нигерии. данный дискриптивно-профильный анализ включал в исследование 300 хирургов-стоматологов, работающих в государственных и частных стоматологических клиниках Нигерии.
Научная работа проводилась с июня 2006 года по январь 2007 года. Подкожные повреждения были выявлены у Saratov Journal of Medical Scientific Research. 2010. Vol. 6. № 2.