WWW.DISSERS.RU


...
    !

C.. Ba EHE CTP KT PHO HK OHA H X MEHEH TE EB OKO O HO O E OHO E E AHA COCTO H HE POTPAHCMTTEPHO PE B C OB X OCTPOO MO OHA HO O EBOO CTPECCA ye pa e c a ana ya ee ne y n ey ee yc x cp a e cpecca. ee a c cc eppacep pey .

ye peae ee aao poa cec ya aa apa. oco- opaoecx oooce cepeop- poe cy ae pc (10 ox), oo e oooyo oeyoo ee axoec ycox ap. cooec ycox ocpoo ooao-oeoo cpec- e cpe opaa eaoco-oo.

ca c oopeeo aao coco x oo- coxec eeca yeoo a- xoepeco epa. ppo, cyap eo c cooecy eco, o cpeccoe pea cya- opo [4]. Aoc aexocepa a oee eceecoe eo aaa ypxocepa opee o eoe opaa ooopa aopa ee apocoo Aopeepa ccepa a cocae yeo cpe [1] ocyecc p aoec cee. Pyca [5]. aexoa c oopax cce, cpe oopx eyee eco eceo poco o eoe aae epa ccea [2]. Tae oeo, o aa [6] c oece aao o oaaoae opee pax opao cepoooepeco aca (Mcce oppyc a peya yce aa- 1a). Pepoyy cocooc e ee oo ea cpecca [3]. yee x o- ee ecoc cea ea e poco ee aoe aee oocoa p- ya c oo 3H-a 3H-ea [7].

ee oppepyx oec, apae- poee epoo-pocoecx cx a oee oecaopo-pcocoe- ceoa aepa cpoa 2,5% yax oooce aex eox cpy- poo aee a S-ooo yepe (pH yp opaa. 7,2-7,4), opaaa 2% pacope OsO4, eMoepoae ooao-oeoo cpec- papoa aeoe opacae oepa ca poo a 45 ecopox pcax-caax aa o-812 [8,9]. paoe cpe acco 180-230 o eoe Desiderato et al. oepa ooy opacpoa ypaa(1974). B ceao aepe ox oep- eao pao ca [10]. Cpe pocaa oec oa co 4 A, ea o- pa epoo pocoe MB 100 A. B opoo oe o oo ye yxoa a a- paoe pec eo opoep [11,12].

opy, pacooey epe aep. o p- p oe cceoa coac paoo paoe ycooo peeca ea- a poee pao c cooae cep, poeoc ocoo axoe eax ox.

pc a aope. oceyee aecee o- Oea peae ee ayopox cx yapo oa (6 A eee 2 ce- o eapex ee ycox ooy) epe o aop copooaoc op- ao-oeoo cpecca, ceye oe, o poae cpeccopo pea y ox, 1 cy oce oopaoo ec cpeccopooycoeo a ae oa ey o aopa pocxo aea aa paoa yco peeco ea eeoc cepeopx eo, ocoeo aeyco oe papaee, a o- peaoo, oopx ycac coxco ape oae epooe- ece pea a ee ea, yeo oo papae. B aepe oe axoc co. B eecx eax ecpye5 aco. Maepa cceoa (pae c ooee 3H-ea.

oooyo oeyoo ee) pa pa- ae opoepecoo cceoa ae cpo oce aepe cpeccopx o- peaoo cepoo oaa yeee cyec (epe 1, 2, 3, 5, 6, 7, 8, 10, 14 cy.), c- apo oa oa paepo ep, a 1 2 "' cee ecpyo eex o, e Aaoa ooeca peopaa o yee. e epy, cae a oapyea eax ox pooo c eppoe cepeopx eo eape- cceyex ee.

x ee, xopoo oapyac p epo- Booee eecx eo c pao-pocoeco ye. p o ap- cpyypo-yoa xapaepca ooecx ee oee paea a oe ceecoa o eepoopo aapx eax oeyoo ee. Oe- oee e eapex ee a cpecaec yeee paepo oeca opae cope oec.

e: epa paypo oaaeco cceoae apeepeco xoepce, oeca o, oxop, o c- eco ea cepeopx eo oaao, o eecye o yce ceecx poec- o cpae c opoo cepe y ox co ayoax. pocxo yeee c- ox oeaec oee opece a cepeopx pay x oae a oe apeepecx cpyyp cpe aoc pacpe aae oaaeco ce, (yao opeeea) aexocepa yee oeca poco, a ae pc epx ooax opy cepeopx oeo, o oxopx, pacoaaxc o p y- oee coa opy ox pooo. Cooco o oc opae. Moxop pe- o ypo ape- xoepeco ea yeceo e opyy opy. x apc yaex eapex eeax, eoxoepoo o c yaca pocee. o oe, o o e e ocoepx B oae cepoo oooyo ee pa.

apy c aao ee caec epe 3-4 cyo oce ec cpeccopoeox peeax epoa ooc ce- o aopa apoax eecx epeopx pay, o e oapyaec a- ax oeaec yeee oeca cepepeaoax. opx pay oae. o copooaeB yeeo pe yoao ao c yeee paepo oxop, aaaapo e oeyoo ee o cpa- e oaaeco ce. Oeaec aye c oooyo eeo oeaec xopoo xae ayo oxop. Heoxoo oepaeoe po aaec peoaa- , o a ao ca cepea, ocoee yxpoaox o a eepoxpoao- o oeyoo eee, aac a yaca, apoee opeeec o- yo, coepae coe oae oee oeco opox oeco. Paep oe oeco cepeopx pay. pe paep eo c pae eppoec y ax aapx eo aa epeee appoa peeax 71-100 3. pecoe ooee, oe aao ac C pyo copo, oeac eece e- e, a aa paypo oaaec c cepeop paya, o c y aa- o ce pacoaac a opao aa oaaeco ce eee payeapo oe oa. poce aao cpae c a o opae- e peo pacpe.

a. Paep ep ax eo appoa Ha ae oeeo, o o cpae c peeax 31-71 3. poe oo, a oapy- pa cpoa oa apexe oeyoe ayo c e cepeop o ee oe, e oooyo, opeepaya, ecpy ee opae c e c coxpaeo cpyypo opaa yee paepe pa o 30 3. B e a yopoae. B aappax ax eo oee pae eepoxpoa- x eax oeyoo ee ecoc oe o apoee opeec e H-ea ocoepo opeeac ee ce opoe oec. Heoxoo oe, e oooyo eee. Ha o ca oe, o apexe oooyo ee ae cepea oeae opacae oaacpeac ayo c paepa ep o 30 ee ecoc e H-a y 3 paa ecpyx ee co- eecx eoo, ocooxc o xpaee cpyypo ooe o cpae cepeopx pay. p o ec eex c opoe e (paep ep 31-70 3), a ep ayoo e oooyo eoeyoo eee peyeceo eppo- ee, e oeyoo eee.

poae (paep ep 71-100 3) coxpa- Cepoooepecoe ee aexoee op (paep ep 31-70 3). ao apeepecx epx ooax o# 1.....

py oex oeo opeeo ee epx pa o cpae c opoe. Heoyee x coepa e ocoepx pa- xoo oe, o apeaoax aae oecex oaaee yaex oe occaoee ypacpyypo opaa eeax. Xoepece epa paoeo oce ec cpeccopoo aopa pocxo cceyex eeax coxpa co cpyyp- cpee, e cepoax co ee.

y opaa. poe opeeeo a- Ha ao ca oa opee aoc aexocepa opy ayco ee e cee ecoc e paa e cpeeo oee co opy - o e 3H-a o opox oaaeox pooo o cpae c opoe pa- e pax eecx eo.

e cae cepea. Apeepece epe ooa opy ce 5-6 cya cepea oeac e- peopx oeo popea ypyo-eeoe aaa, a ae poecc opaa op- ceee e apoe pacpe o coaa yoao eeoc cepeop- ey xoy. poe opeee ooao ax eeo eapex ee. Ha o- peepecx cpyypax e oaec o aoeeo opacae ecoc coxec- oo y ax ox. Xoepece epx pea a ee ea oae, oco- a cceyex eeax coxpa co eo cepoax oooyo ee, ce- cpyypy opaa. Aoc aexoe p o ecoc pea a opeee- -cepa opy ayco yao opeee -ox cycpao cepeopx ac a cpe a oee coo opy pay o ypo aox y ax ox. ox pooo.

pe poecc ce xapaepa cepe oee oyee peya ceecy o oeo poceaec aapx eax pax ooocx aaa oeca oooyo ee, e oeyoo ee- apyex y eo eapex ee. Oeeo ae cee ecoc e o- oepaoo pocxoe, oe paoao e 3H-ea oo- ax p ec cpeccopx aopo. Pepeeo a cepoax, a apeaoax opaa e oooyo ee poo oaaee opoo cep. ac paeoc poecco ce xapaepa p epoo-pocoeco cceoa- cepe (eooo a cc). Opeee o eecx eo opeeeo, o epo- oe poecco peao cpyypo ea ooc xapaep pacooe cepeop- pecpo eo oeyoo eee. yex pay oae, paep epa o- e xoe cepea peae ee aaeco ce acaoo oeca aao co- opaoecx oe o, oeco cax poco cao- e eapex ee ca c poeccac a e, a y ax ox. Te e cpyypx ee ape- xoepeceee, y apoax eox op oxo- o ea cceyex oeax. B coe p yee, oao ayxe, pc x cooyoc peee a oo epao ac opae papyac. B pax yo cyecyy epooo oecoooee y- eepoxpoaox o co o oo ooa- xoepeco oopox oeco apoe e e oco- poe eooo aeoo oeocaa.

_ Cco cooao epayp:

1. Selye H. The story of the adaptation syndrom. Montreal. 1952. 225 p.

2. Aox.. oo epooo ycooo peeca. M.: Mea, 1968. 547 c.

3. Meepo .. ae e aaa eoope epce pa aaaoo e // c. o. ay, 1991, T. 22. N2. C. 52-89.

4. pc . cox eopeeca paa. M.: -o ocp. epayp, 1962. 962 c.

5. Karnovsky M.J. Roots L. A directcolloring Thiocholine method for cholinesterase // J. Histochem. Cytochem. 1964. vol. 12. p. 219-221.

6. Falck B.N. Observations on the possibilities of the cellular localization of monoamines by a fluorescence method // Acta physiol. Scand.

1962.vol. 56, sappl.197. p. 1-25.

7. .H. peee paoax ooo coo // Paoae aop coo. .: -o M AMH CCCP, 1959. C. 5-33.

8. Millonig G. The advantages of a phosphate buffer for 0s04 solutions in fixation // J. appl. physics. 1961. vol.32. p. 1637-1638.

9. Sato T., Shamoto M. A simple rapid polychrome stain for epoxyembedded tissue. Stain Technol., 1973. v. 48. p. 223.

10. Reynolds E.S. The use of lead citrate at high pH as an electronopaque stain in electron microscopy // J. Cell Biol. 1963. vol. 17. p. 203-213.

11.Aao.. Meca opoep. M. : Mea, 1990. 384 c.

12.Tae . Beee oecey ocooecy opoo.

yxapec: -o Aae CPP, 1980. 191 c.




2011 www.dissers.ru -

, .
, , , , 1-2 .